Efficient training of mice on the 5-choice serial reaction time task in an automated rodent training system.
Efficient training of mice on the 5-choice serial reaction time task in an automated rodent training system.
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DOI：
10.1038/s41598-020-79290-2
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发表时间：
2020-12-21
影响因子：
4.6
通讯作者：
Hangya B
中科院分区：
文献类型：
--
作者：
Birtalan E;Bánhidi A;Sanders JI;Balázsfi D;Hangya B
旨在了解感觉运动系统、认知和行为的实验需要训练动物执行复杂的任务。传统的训练方案要求实验室人员在家庭笼和训练室之间移动动物，开始和结束训练课程，并且在某些情况下，手动控制每个训练试验。人类劳动不仅限制了每天的训练量，而且还引入了几个可变性来源，并可能增加动物的压力。在这里，我们提出了一个自动化的训练系统的5选择系列反应时间任务（5CSRTT），一个经典的啮齿动物的任务，经常用来测试感官检测，持续的注意力和冲动。我们发现，在没有人为干预的情况下，完全自动化可以实现快速，具有成本效益的培训，并通过皮质酮水平来降低压力。训练中断仅引起表现的短暂下降，并且当从自动化方案转移到手动方案时，小鼠容易在训练系统中泛化。我们通过无线光遗传学和药理学实验进一步验证了我们的自动化训练系统，扩大了我们系统可以满足的实验需求的广度。我们的自动化5CSRTT系统可以作为全自动行为训练的原型，其方法和原理可转移到一系列啮齿动物任务中。
Experiments aiming to understand sensory-motor systems, cognition and behavior necessitate training animals to perform complex tasks. Traditional training protocols require lab personnel to move the animals between home cages and training chambers, to start and end training sessions, and in some cases, to hand-control each training trial. Human labor not only limits the amount of training per day, but also introduces several sources of variability and may increase animal stress. Here we present an automated training system for the 5-choice serial reaction time task (5CSRTT), a classic rodent task often used to test sensory detection, sustained attention and impulsivity. We found that full automation without human intervention allowed rapid, cost-efficient training, and decreased stress as measured by corticosterone levels. Training breaks introduced only a transient drop in performance, and mice readily generalized across training systems when transferred from automated to manual protocols. We further validated our automated training system with wireless optogenetics and pharmacology experiments, expanding the breadth of experimental needs our system may fulfill. Our automated 5CSRTT system can serve as a prototype for fully automated behavioral training, with methods and principles transferrable to a range of rodent tasks.
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影响因子：
--
作者：
Higgins, Guy A;Silenieks, Leo B
通讯作者：
Silenieks, Leo B
影响因子：
3.7
作者：
Goltstein PM;Reinert S;Glas A;Bonhoeffer T;Hübener M
通讯作者：
Hübener M
DOI：
10.1080/10253890.2017.1423053
发表时间：
2018-01-01
影响因子：
2.3
作者：
Balazsfi, Diana;Fodor, Anna;Zelena, Dora
通讯作者：
Zelena, Dora
影响因子：
3.7
作者：
Easton, A;Ridley, RM;Gaffan, D
通讯作者：
Gaffan, D
中央胆碱能神经元被增强反馈迅速招募。
影响因子：
64.5
作者：
Hangya B;Ranade SP;Lorenc M;Kepecs A
通讯作者：
Kepecs A