Antioxidant activities of uyaku (lindera strychnifolia) leaf extract: a natural extract used in traditional medicine.
Antioxidant activities of uyaku (lindera strychnifolia) leaf extract: a natural extract used in traditional medicine.
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DOI：
10.3164/jcbn2007019
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发表时间：
2007-09
影响因子：
2.4
通讯作者：
Mori A
中科院分区：
文献类型：
--
作者：
Noda Y;Mori A
[结果]山胡椒（Lindera strychnifolia，Sieb. et Zucc.）在亚洲传统医学中用于治疗胃和肾脏疾病、神经痛、风湿和衰老。在这项研究中，冻干提取物对羟基（·OH）和超氧（O2·−）自由基的影响，使用电子自旋共振（ESR）光谱仪与自旋陷阱，5，5 '-二甲基-1-吡咯啉-N-氧化物。使用以下试剂评估抑制作用：对于一氧化氮（NO·），使用Griess试剂;对于（Fe 2 + + H2 O2）诱导的脂质过氧化，使用2-硫代巴比妥酸;对于（Fe 2 + + H2 O2）诱导的蛋白质羰基，使用2，4-二硝基苯肼。提取物的ESR数据分析表明直接清除·OH和O2·−。该提取物能剂量依赖性地清除NO·，抑制亚麻酸脂质过氧化和牛血清白蛋白羰基化。结果表明，乌叶热水提取物具有较强的活性氧和活性氮清除能力，并能有效抑制脂质过氧化反应。这些结果可能有助于理解与年龄相关的或自由基相关的疾病诱导过量的活性氧和氮物种。
Uyaku (Lindera strychnifolia, Sieb. et Zucc.) is used in traditional Asian medicine to treat stomach and renal diseases, neuralgia, rheumatism, and aging. In this study, the effects of lyophilized extracts on hydroxyl (·OH) and superoxide (O2·−) radicals were examined using an electron spin resonance (ESR) spectrometer with the spin trap, 5,5'-dimethyl-1-pyrroline-N-oxide. Inhibitory effects were assessed using the following reagents: for nitric oxide (NO·), the Griess reagent; for (Fe2+ + H2O2)-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe2+ + H2O2)-induced protein carbonyl, 2,4-dinitrophenylhydrazine. Analysis of ESR data of the extracts indicated the direct ·OH and O2·− scavenging. The extracts scavenged NO· in a dose-dependent manner, inhibited lipid peroxidation of linolenic acid, and protein carbonyl formation in bovine serum albumin. In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation. These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.
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DOI：
10.1016/s1357-2725(96)00144-6
发表时间：
1997-03-01
影响因子：
4
作者：
Kimura, K
通讯作者：
Kimura, K
影响因子：
6.1
作者：
Ohno, T;Takemura, G;Fujiwara, H
通讯作者：
Fujiwara, H
影响因子：
5.7
作者：
Li, YM;Ohno, Y;Fujiwara, H
通讯作者：
Fujiwara, H
影响因子：
2
作者：
Kobayashi, W;Miyase, T;Noguchi, H
通讯作者：
Noguchi, H
影响因子：
--
作者：
LAGERCRANTZ, C
通讯作者：
LAGERCRANTZ, C