Molecular characterization of clinical isolates of Aeromonas species from Malaysia.
Molecular characterization of clinical isolates of Aeromonas species from Malaysia.
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DOI：
10.1371/journal.pone.0030205
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Chua KH
中科院分区：
文献类型：
--
作者：
Puthucheary SD;Puah SM;Chua KH
气单胞菌是水生环境的常见居民，可引起鱼类和人类的感染。由于其表型和基因型异质性，气单胞菌在物种水平上的鉴定是有问题和复杂的。利用先前发表的针对GCAT、16S rDNA和rpoD基因的方法，对嗜水气单胞菌或气单胞菌进行基因重新鉴定。经GCAT-PCR鉴定，98株菌株中94株（96%）属于气单胞菌属。采用16S rDNA-RFLP方法对94株菌株进行鉴定，鉴定出3个聚类，分别为A. caviae（61%）、A. hydrophila（17%）和未知聚类（22%），其RFLP限制模式不典型。然而，利用rpoD序列构建的系统发育树显示，有47株（50%）与水浒类型菌株聚集在一起，18株（19%）与caviae聚集在一起，16株（17%）与A. hydroila聚集在一起，12株（13%）与A. veronii聚集在一起，1株（1%）与A. trota类型菌株聚集在一起。PCR结果显示，94株菌株中存在10个毒力基因，分别为lip（91%）、exu（87%）、ela（86%）、alt（79%）、ser（77%）、fla（74%）、aer（72%）、act（43%）、aexT（24%）和ast（23%）。本研究强调了使用多种方法正确鉴定气单胞菌菌株的重要性。rpoD基因的序列使94株气单胞菌的分离得到明确的鉴定，与最近其他研究结果一致。水族气单胞菌（Aeromonas aquariorum）是最常见的菌种（50%），含有重要的毒力基因lip/alt/ser/fla/aer亚群。菌株中存在的不同毒力基因组合表明它们可能在气单胞菌感染的发病机制中起作用。
Aeromonas species are common inhabitants of aquatic environments giving rise to infections in both fish and humans. Identification of aeromonads to the species level is problematic and complex due to their phenotypic and genotypic heterogeneity. Aeromonas hydrophila or Aeromonas sp were genetically re-identified using a combination of previously published methods targeting GCAT, 16S rDNA and rpoD genes. Characterization based on the genus specific GCAT-PCR showed that 94 (96%) of the 98 strains belonged to the genus Aeromonas. Considering the patterns obtained for the 94 isolates with the 16S rDNA-RFLP identification method, 3 clusters were recognised, i.e. A. caviae (61%), A. hydrophila (17%) and an unknown group (22%) with atypical RFLP restriction patterns. However, the phylogenetic tree constructed with the obtained rpoD sequences showed that 47 strains (50%) clustered with the sequence of the type strain of A. aquariorum, 18 (19%) with A. caviae, 16 (17%) with A. hydrophila, 12 (13%) with A. veronii and one strain (1%) with the type strain of A. trota. PCR investigation revealed the presence of 10 virulence genes in the 94 isolates as: lip (91%), exu (87%), ela (86%), alt (79%), ser (77%), fla (74%), aer (72%), act (43%), aexT (24%) and ast (23%). This study emphasizes the importance of using more than one method for the correct identification of Aeromonas strains. The sequences of the rpoD gene enabled the unambiguous identication of the 94 Aeromonas isolates in accordance with results of other recent studies. Aeromonas aquariorum showed to be the most prevalent species (50%) containing an important subset of virulence genes lip/alt/ser/fla/aer. Different combinations of the virulence genes present in the isolates indicate their probable role in the pathogenesis of Aeromonas infections.
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影响因子：
9.4
作者：
Aravena-Roman, Max;Harnett, Gerald B.;Chang, Barbara J.
通讯作者：
Chang, Barbara J.
DOI：
10.1099/ijs.0.65352-0
发表时间：
2008-05-01
影响因子：
2.8
作者：
Martinez-Murcia, A. J.;Saavedra, M. J.;Cousin, S.
通讯作者：
Cousin, S.
影响因子：
3.4
作者：
Beaz-Hidalgo, Roxana;Alperi, Anabel;Jose Figueras, Maria
通讯作者：
Jose Figueras, Maria
影响因子：
3.1
作者：
Alperi, Anabel;Figueras, Maria J.;Martinez-Murcia, Antonio J.
通讯作者：
Martinez-Murcia, Antonio J.
影响因子：
9.4
作者：
Borrell, N;Acinas, SG;MartinezMurcia, AJ
通讯作者：
MartinezMurcia, AJ