FISH-Based Markers Enable Identification of Chromosomes Derived From Tetraploid Thinopyrum elongatum in Hybrid Lines.
FISH-Based Markers Enable Identification of Chromosomes Derived From Tetraploid Thinopyrum elongatum in Hybrid Lines.
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基于 FISH 的标记能够鉴定杂交系中四倍体 Thinopyrum elongatum 衍生的染色体
DOI：
10.3389/fpls.2018.00526
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发表时间：
2018
影响因子：
5.6
通讯作者：
Kang H
中科院分区：
文献类型：
--
作者：
Li D;Li T;Wu Y;Zhang X;Zhu W;Wang Y;Zeng J;Xu L;Fan X;Sha L;Zhang H;Zhou Y;Kang H
四倍体长穗偃麦草具有优良的非生物耐逆性，对条锈病、白粉病和赤霉病具有较强的抗性，是小麦的野生近缘种，是小麦改良新基因的重要来源。目前，一种高分辨率荧光原位杂交(FISH)核型的四倍体Th。不提供拉格特姆。为了开发染色体特异的FISH标记，以六倍体小黑麦8801和两个四倍体Th.利用不同重复序列的探针对长梗进行了鉴定。我们发现，利用pSc119.2、pTa535、pTa71和pTa713重复序列的组合可以明确地识别所有的E-基因组染色体，而野生材料和部分两倍体的E-基因组染色体在探针杂交模式上没有表现出任何显著的变化。为了验证这些标记的有效性，对8个小麦-Th.对拉格坦杂交种的衍生物进行了分析。我们发现这些探针可以快速检测到小麦和四倍体Th。杂交种的细长染色体。K16-712-1-2为1E(1D)染色体置换系，K16-681-4为2E二体染色体附加系，K16-562-3为3E，4E(3D，4D)染色体置换系，K15-1033-8-2含有1条4E、2条5E和1条4ES⋅1d1罗伯逊易位染色体，另外4株分别携带单体4E、5E、6E和7E染色体。E基因组特异性分子标记分析结果与FISH结果吻合较好。所开发的FISH标记将有助于四倍体Th的快速鉴定。在小麦改良计划中使用拉长染色体，并允许适当的异源染色体转移。
Tetraploid Thinopyrum elongatum, which has superior abiotic stress tolerance characteristics, and exhibits resistance to stripe rust, powdery mildew, and Fusarium head blight, is a wild relative of wheat and a promising source of novel genes for wheat improvement. Currently, a high-resolution Fluorescence in situ hybridization (FISH) karyotype of tetraploid Th. elongatum is not available. To develop chromosome-specific FISH-based markers, the hexaploid Trititrigia 8801 and two accessions of tetraploid Th. elongatum were characterized by different repetitive sequences probes. We found that all E-genome chromosomes could be unambiguously identified using a combination of pSc119.2, pTa535, pTa71, and pTa713 repeats, and the E-genome chromosomes of the wild accessions and the partial amphiploid failed to exhibit any significant variation in the probe hybridization patterns. To verify the validation of these markers, the chromosome constitution of eight wheat- Th. elongatum hybrid derivatives were analyzed. We revealed that these probes could quickly detect wheat and tetraploid Th. elongatum chromosomes in hybrid lines. K16-712-1-2 was a 1E (1D) chromosome substitution line, K16-681-4 was a 2E disomic chromosome addition line, K16-562-3 was a 3E, 4E (3D, 4D) chromosome substitution line, K15-1033-8-2 contained one 4E, two 5E, and one 4ES⋅1DL Robertsonian translocation chromosome, and four other lines carried monosomic 4E, 5E, 6E, and 7E chromosome, respectively. Furthermore, the E-genome specific molecular markers analysis corresponded perfectly with the FISH results. The developed FISH markers will facilitate rapid identification of tetraploid Th. elongatum chromosomes in wheat improvement programs and allow appropriate alien chromosome transfer.
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影响因子：
3.1
作者：
Fedak, G
通讯作者：
Fedak, G
影响因子：
5.4
作者：
Cuadrado, A;Vitellozzi, F;Ceoloni, C
通讯作者：
Ceoloni, C
影响因子：
3.1
作者：
Dai, Yi;Duan, Yamei;Chen, Jianmin
通讯作者：
Chen, Jianmin
影响因子：
2.6
作者：
Alkhimova, AG;Heslop-Harrison, JS;Vershinin, AV
通讯作者：
Vershinin, AV
DOI：
10.1073/pnas.85.11.3805
发表时间：
1988-06-01
影响因子：
11.1
作者：
DVORAK, J;EDGE, M;ROSS, K
通讯作者：
ROSS, K