Cholinergic synaptic transmissions were altered after single sevoflurane exposure in Drosophila pupa.
Cholinergic synaptic transmissions were altered after single sevoflurane exposure in Drosophila pupa.
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DOI：
10.1155/2015/485709
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发表时间：
2015
影响因子：
--
通讯作者：
Gu H
中科院分区：
文献类型：
--
作者：
Chen R;Zhang T;Kuang L;Chen Z;Ran D;Niu Y;Xu K;Gu H
目的.七氟醚是目前临床上应用最广泛的全身麻醉药之一。既往研究表明七氟醚可诱导神经元凋亡和神经功能缺损，引起对七氟醚麻醉安全性的质疑。采用全脑记录技术研究七氟醚对以乙酰胆碱为兴奋性神经递质的果蝇蛹的电生理的影响。方法.将广种果蝇随机分为对照组和七氟醚组。七氟醚组（1%七氟醚、2%七氟醚、3%七氟醚）分别暴露于七氟醚，暴露持续5 h。暴露后24 h，采用膜片钳技术进行电生理学实验。结果结果表明，七氟醚暴露后24小时，微兴奋性突触后电流（mEPSCs）的频率显著降低（P < 0.05），但幅度无显著变化。此外，我们还探讨了其可能的机制，发现七氟醚暴露后，部分调节mEPSCs频率的钙电流密度显著降低（P < 0.05）。结论.提示七氟醚可通过调节钙通道改变与突触可塑性相关的mEPSCs。
Purpose. Sevoflurane, one of the most used general anesthetics, is widely used in clinical practice all over the world. Previous studies indicated that sevoflurane could induce neuron apoptosis and neural deficit causing query in the safety of anesthesia using sevoflurane. The present study was designed to investigate the effects of sevoflurane on electrophysiology in Drosophila pupa whose excitatory neurotransmitter is acetylcholine early after sevoflurane exposure using whole brain recording technique. Methods. Wide types of Drosophila (canton-s flies) were allocated to control and sevoflurane groups randomly. Sevoflurane groups (1% sevoflurane; 2% sevoflurane; 3% sevoflurane) were exposed to sevoflurane and the exposure lasted 5 hours, respectively. All flies were subjected to electrophysiology experiment using patch clamp 24 hours after exposure. Results. The results showed that, 24 hours after sevoflurane exposure, frequency but not the amplitude of miniature excitatory postsynaptic currents (mEPSCs) was significantly reduced (P < 0.05). Furthermore, we explored the underlying mechanism and found that calcium currents density, which partially regulated the frequency of mEPSCs, was significantly reduced after sevoflurane exposure (P < 0.05). Conclusions. All these suggested that sevoflurane could alter the mEPSCs that are related to synaptic plasticity partially through modulating calcium channel early after sevoflurane exposure.
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影响因子：
16.2
作者：
Ng, M;Roorda, RD;Miesenböck, G
通讯作者：
Miesenböck, G
影响因子：
3.6
作者：
Kostopanagiotou, Georgia;Kalimeris, Konstantinos;Papageorgiou, Charalambos
通讯作者：
Papageorgiou, Charalambos
急性低剂量三聚氰胺影响大鼠海马突触可塑性和行为
影响因子：
3.5
作者：
Yang, Yuan;Xiong, Gui-Jing;Mao, Rong-Rong
通讯作者：
Mao, Rong-Rong
影响因子：
8.8
作者：
Liang G;Ward C;Peng J;Zhao Y;Huang B;Wei H
通讯作者：
Wei H
影响因子：
3.1
作者：
Amaral MD;Pozzo-Miller L
通讯作者：
Pozzo-Miller L