Characterization of the expressed gene and several processed pseudogenes for the mouse ribosomal protein L30 gene family

Characterization of the expressed gene and several processed pseudogenes for the mouse ribosomal protein L30 gene family
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小鼠核糖体蛋白 L30 基因家族表达基因和几个加工假基因的表征

DOI:
10.1128/mcb.4.11.2518-2528.1984
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发表时间:
1984
影响因子:
5.3
通讯作者:
R. Perry
R. Perry
中科院分区:
生物学2区
文献类型:
--
作者:
L. Wiedemann;R. Perry

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从重组DNA文库中分离到5个编码小鼠核糖体蛋白L30的克隆基因,并对其进行限制性内切定位和核苷酸序列分析。这些基因中只有一个含有内含子并被表达;其他的是不活跃的加工假基因。所表达的基因由5个外显子和4个内含子组成,跨越2,723个核苷酸。该基因的转录本被加工成成熟的L30 mRNA,其途径在内含子切除的顺序方面表现出限制和灵活性。l30mrna的长度为457 ~ 468个核苷酸,不包括聚腺苷酸尾部,在其3'端表现出一定的微观异质性,编码115个氨基酸的基本蛋白质。rpL30基因的5'部分具有一些与先前表征的小鼠rpL32基因非常相似的新特征。这些包括-60至-340区域的同源序列,缺乏良好的TATA共识序列,以及存在跨越帽位的回文嘧啶序列。
Five cloned genes encoding the mouse ribosomal protein L30 were isolated from a recombinant DNA library and characterized by restriction mapping and nucleotide sequence analysis. Only one of these genes has introns and is expressed; the others are inactive processed pseudogenes. The expressed gene consists of five exons and four introns spanning 2,723 nucleotides. Transcripts of this gene are processed into the mature L30 mRNA by pathways that exhibit both constraints and flexibility with regard to the order of intron excision. The L30 mRNA which is 457 to 468 nucleotides in length excluding the polyadenylic acid tail, exhibits some microheterogeneity at its 3' end and encodes a basic protein of 115 amino acids. The 5' portion of the rpL30 gene has some novel features which are remarkably similar to the previously characterized mouse rpL32 gene. These include homologous sequences in the -60 to -340 region, the absence of a good TATA consensus sequence, and the presence of a palindromic pyrimidine sequence that spans the cap site.
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