Nucleic Acid aptamers: new methods for selection, stabilization, and application in biomedical science.
Nucleic Acid aptamers: new methods for selection, stabilization, and application in biomedical science.
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DOI：
10.4062/biomolther.2013.085
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发表时间：
2013-11
影响因子：
3.7
通讯作者：
Byun J
中科院分区：
文献类型：
--
作者：
Kong HY;Byun J
寡核苷酸适体的采用正在不断增加，满足了生物医学领域对多功能性不断增长的需求。通过SELEX（指数富集的配体系统进化），可以获得能够以高亲和力和特异性结合特定靶标的适配体。适体是单链核酸分子，可以折叠成复杂的三维结构，形成结合袋和裂缝，用于特异性识别和紧密结合任何给定的分子靶标。近年来，适配体因其不仅具有抗体的所有优点，而且具有热稳定性、易于合成、可逆性、免疫原性小等独特优点而备受关注。新技术的出现正在彻底改变适配体的应用。适体可以很容易地通过各种化学反应进行修饰，以引入官能团和/或核苷酸延伸。它们还可以与治疗分子缀合，例如药物、含有药物的载体、毒素或光敏剂。在这里，我们讨论新的 SELEX 策略和稳定方法以及在药物输送和分子成像中的应用。
The adoption of oligonucleotide aptamer is well on the rise, serving an ever increasing demand for versatility in biomedical field. Through the SELEX (Systematic Evolution of Ligands by EXponential enrichment), aptamer that can bind to specific target with high affinity and specificity can be obtained. Aptamers are single-stranded nucleic acid molecules that can fold into complex threedimensional structures, forming binding pockets and clefts for the specific recognition and tight binding of any given molecular target. Recently, aptamers have attracted much attention because they not only have all of the advantages of antibodies, but also have unique merits such as thermal stability, ease of synthesis, reversibility, and little immunogenicity. The advent of novel technologies is revolutionizing aptamer applications. Aptamers can be easily modified by various chemical reactions to introduce functional groups and/or nucleotide extensions. They can also be conjugated to therapeutic molecules such as drugs, drug containing carriers, toxins, or photosensitizers. Here, we discuss new SELEX strategies and stabilization methods as well as applications in drug delivery and molecular imaging.
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影响因子：
6.7
作者：
Cui, Zong-Qiang;Ren, Qian;Zhang, Xian-En
通讯作者：
Zhang, Xian-En
CS-SELEX产生高亲和力的ssDNA适体作为乙型肝炎病毒包膜糖蛋白E2的分子探针。
影响因子：
3.7
作者：
Chen F;Hu Y;Li D;Chen H;Zhang XL
通讯作者：
Zhang XL
影响因子：
2.9
作者：
Cox, JC;Rudolph, P;Ellington, AD
通讯作者：
Ellington, AD
DOI：
10.1046/j.1432-1327.2000.01557.x
发表时间：
2000-08-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Andreola, ML;Calmels, C;Litvak, S
通讯作者：
Litvak, S
影响因子：
3.5
作者：
Holland, CA;Henry, AT;Church, FC
通讯作者：
Church, FC