Assessing Aedes aegypti candidate genes during viral infection and Wolbachia-mediated pathogen blocking.
Assessing Aedes aegypti candidate genes during viral infection and Wolbachia-mediated pathogen blocking.
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DOI：
10.1111/imb.12764
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发表时间：
2022-06
影响因子：
2.6
通讯作者：
中科院分区：
文献类型：
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控制登革热病毒传播的一种方法是共生体Wolbachia，它限制了蚊子的病毒感染。尽管有计划将其广泛用于埃及伊蚊，但对沃尔巴克氏体的作用方式仍知之甚少。许多研究表明，这一机制可能是多方面的，涉及免疫、细胞应激和营养竞争等方面。我们小组先前的一项研究使用人工选择来鉴定一种与病毒阻断相关的新蚊子候选基因; α-甘露糖苷酶-2a（alpha-Mann-2a），其在蛋白质糖基化中具有预测作用。蛋白质糖基化途径往往参与复杂的宿主-病毒相互作用;然而，α-甘露聚糖酶的功能尚未在蚊子-病毒相互作用中描述。我们检测了α-Mann-2a对病毒和沃尔巴克氏体感染的反应，以及RNA干扰引起的基因表达降低是否会影响病毒载量。我们发现登革病毒（DENV）感染以组织和时间依赖性方式影响α-Mann-2a的表达，而Wolbachia感染则没有影响。在中肠中，在无沃尔巴克氏体的蚊子中敲低α-Mann-2a表达后，DENV患病率增加，表明α-Mann-2a干扰感染。表达敲低对披膜病毒基孔肯雅病毒具有相同的作用，表明α-Mann-2a可能在中肠中具有广泛的抗病毒作用。有趣的是，我们无法敲低沃尔巴克氏体感染蚊子中的表达。我们还提供了证据表明，α-Mann-2a可能会影响另一个预测参与病毒阻断和细胞粘附的基因的转录水平;钙粘蛋白87 a。这些数据支持的假设，糖基化和粘附途径可能广泛参与了病毒感染的Ae。埃及人。埃及伊蚊候选Wolbachia介导的病原体阻断基因α-Mann-2a的表达以组织和时间依赖性方式受到登革热病毒（DENV）的影响，Wolbachia感染没有影响。在中肠中α-Mann-2a表达敲低后，DENV感染率在无沃尔巴克氏体的蚊子中增加，在基孔肯雅病毒感染期间观察到相同的效果。α-Mann-2a表达的敲低也降低了另一个候选Wolbachia介导的病原体阻断基因cadherin 87 a，这表明这两个基因都有助于感染结果。
One approach to control dengue virus transmission is the symbiont Wolbachia, which limits viral infection in mosquitoes. Despite plans for its widespread use in Aedes aegypti, Wolbachia's mode of action remains poorly understood. Many studies suggest that the mechanism is likely multifaceted, involving aspects of immunity, cellular stress and nutritional competition. A previous study from our group used artificial selection to identify a new mosquito candidate gene related to viral blocking; alpha‐mannosidase‐2a (alpha‐Mann‐2a) with a predicted role in protein glycosylation. Protein glycosylation pathways tend to be involved in complex host–viral interactions; however, the function of alpha‐mannosidases has not been described in mosquito–virus interactions. We examined alpha‐Mann‐2a expression in response to virus and Wolbachia infections and whether reduced gene expression, caused by RNA interference, affected viral loads. We show that dengue virus (DENV) infection affects the expression of alpha‐Mann‐2a in a tissue‐ and time‐dependent manner, whereas Wolbachia infection had no effect. In the midgut, DENV prevalence increased following knockdown of alpha‐Mann‐2a expression in Wolbachia‐free mosquitoes, suggesting that alpha‐Mann‐2a interferes with infection. Expression knockdown had the same effect on the togavirus chikungunya virus, indicating that alpha‐Mann‐2a may have broad antivirus effects in the midgut. Interestingly, we were unable to knockdown the expression in Wolbachia‐infected mosquitoes. We also provide evidence that alpha‐Mann‐2a may affect the transcriptional level of another gene predicted to be involved in viral blocking and cell adhesion; cadherin87a. These data support the hypothesis that glycosylation and adhesion pathways may broadly be involved in viral infection in Ae. aegypti. The expression of an Aedes aegypti candidate Wolbachia‐mediated pathogen blocking gene, alpha‐Mann‐2a, is affected by dengue virus (DENV) in a tissue‐ and time‐dependent manner, and Wolbachia infection had no effect. Following knockdown of alpha‐Mann‐2a expression in the midgut, DENV infection prevalence increased in Wolbachia‐free mosquitoes, and the same effect was observed during chikungunya virus infection. Knockdown of alpha‐Mann‐2a expression also decreases another candidate Wolbachia‐mediated pathogen blocking gene, cadherin87a, suggesting that both genes contribute to infection outcomes.
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影响因子：
3.8
作者：
Brustolin M;Pujhari S;Henderson CA;Rasgon JL
通讯作者：
Rasgon JL
影响因子：
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作者：
Muñoz Mde L;Limón-Camacho G;Tovar R;Diaz-Badillo A;Mendoza-Hernández G;Black WC 4th
通讯作者：
Black WC 4th
影响因子：
3.8
作者：
Caragata EP;Rezende FO;Simões TC;Moreira LA
通讯作者：
Moreira LA
影响因子：
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作者：
Banerjee N;Mukhopadhyay S
通讯作者：
Mukhopadhyay S
影响因子：
3.8
作者：
Dutra HLC;Ford SA;Allen SL;Bordenstein SR;Chenoweth SF;Bordenstein SR;McGraw EA
通讯作者：
McGraw EA