Identification and Expression Patterns of Putative Diversified Carboxylesterases in the Tea Geometrid Ectropis obliqua Prout.
Identification and Expression Patterns of Putative Diversified Carboxylesterases in the Tea Geometrid Ectropis obliqua Prout.
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DOI：
10.3389/fphys.2017.01085
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发表时间：
2017
影响因子：
4
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Sun L;Wang Q;Wang Q;Zhang Y;Tang M;Guo H;Fu J;Xiao Q;Zhang Y;Zhang Y
羧酸酯酶 (CXE) 属于代谢酶家族。一些CXE充当气味降解酶（ODE），据报道它们在昆虫嗅觉器官中高表达，并参与酯信息素成分和植物挥发物的快速失活。茶尺蠖 Ectropis obliqua Prout 产生由非酯功能化合物组成的性信息素，但严重依赖乙酸酯植物挥发物来寻找寄主植物并定位产卵位点。然而，表征这种重要茶树害虫的假定候选 ODE 的研究仍然相对较少。在本研究中，我们根据先前获得的转录组数据，从 E. obliqua 化学感应器官中鉴定了 35 个候选 EoblCXE 基因。推导的氨基酸序列具有昆虫CXE家族的典型特征，包括氧阴离子孔残基、Ser-Glu-His催化三联体以及酯酶保守五肽特征中包含的Ser活性Gly-X-Ser-X-Gly。系统发育分析表明，EoblCXE 具有多样性，属于几个不同的昆虫酯酶进化枝。通过逆转录和定量实时聚合酶链反应分析（RT-和qRT-PCR）研究了组织和性别相关的表达模式。结果表明，35个EoblCXE基因呈现出多样化的表达谱；其中，12个EoblCXE似乎是触角偏向的，2个EoblCXE是非化学感应器官偏向的，12个EoblCXE是普遍存在的，9个EoblCXE在不同组织中表现出异质的表达水平。有趣的是，两个 EoblCXE 基因 EoblCXE7 和 EoblCXE13 不仅强烈定位于针对气味剂调节的触角感觉器，例如毛感器（Str I 和 II）和基本感器（Sba），而且还在假定的味觉茎锥感器（Sst）中表达，表明这两个 CXE 可能在E. obliqua化学感应处理系统。这项研究首次阐明了尺蛾化学感应系统中的 CXE，并将有助于更全面地了解跨鳞翅目昆虫 CXE 的功能。
Carboxylesterases (CXEs) belong to a family of metabolic enzymes. Some CXEs act as odorant-degrading enzymes (ODEs), which are reportedly highly expressed in insect olfactory organs and participate in the rapid deactivation of ester pheromone components and plant volatiles. The tea geometrid Ectropis obliqua Prout produces sex pheromones consisting of non-ester functional compounds but relies heavily on acetic ester plant volatiles to search for host plants and locate oviposition sites. However, studies characterizing putative candidate ODEs in this important tea plant pest are still relatively scarce. In the present study, we identified 35 candidate EoblCXE genes from E. obliqua chemosensory organs based on previously obtained transcriptomic data. The deduced amino acid sequences possessed the typical characteristics of the insect CXE family, including oxyanion hole residues, the Ser-Glu-His catalytic triad, and the Ser active included in the conserved pentapeptide characteristic of esterases, Gly-X-Ser-X-Gly. Phylogenetic analyses revealed that the EoblCXEs were diverse, belonging to several different insect esterase clades. Tissue- and sex-related expression patterns were studied via reverse-transcription and quantitative real-time polymerase chain reaction analyses (RT- and qRT-PCR). The results showed that 35 EoblCXE genes presented a diversified expression profile; among these, 12 EoblCXEs appeared to be antenna-biased, two EoblCXEs were non-chemosensory organ-biased, 12 EoblCXEs were ubiquitous, and nine EoblCXEs showed heterogeneous expression levels among different tissues. Intriguingly, two EoblCXE genes, EoblCXE7 and EoblCXE13, were not only strongly localized to antennal sensilla tuned to odorants, such as the sensilla trichodea (Str I and II) and sensilla basiconica (Sba), but were also expressed in the putative gustatory sensilla styloconica (Sst), indicating that these two CXEs might play multiple physiological roles in the E. obliqua chemosensory processing system. This study provides the first elucidation of CXEs in the chemosensory system of a geometrid moth species and will enable a more comprehensive understanding of the functions of insect CXEs across lepidopteran species.
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影响因子：
3.7
作者：
Durand N;Carot-Sans G;Bozzolan F;Rosell G;Siaussat D;Debernard S;Chertemps T;Maïbèche-Coisne M
通讯作者：
Maïbèche-Coisne M
影响因子：
3.8
作者：
Ishida, Y;Leal, WS
通讯作者：
Leal, WS
DOI：
10.1073/pnas.0505340102
发表时间：
2005-09-27
影响因子：
11.1
作者：
Ishida, Y;Leal, WS
通讯作者：
Leal, WS
影响因子：
5.4
作者：
Chertemps T;François A;Durand N;Rosell G;Dekker T;Lucas P;Maïbèche-Coisne M
通讯作者：
Maïbèche-Coisne M
DOI：
10.1016/0020-1790(87)90093-x
发表时间：
1987-01-01
期刊：
INSECT BIOCHEMISTRY
影响因子：
--
作者：
KLEIN, U
通讯作者：
KLEIN, U