Deep sympatric mitochondrial divergence without reproductive isolation in the common redstart Phoenicurus phoenicurus.
Deep sympatric mitochondrial divergence without reproductive isolation in the common redstart Phoenicurus phoenicurus.
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DOI：
10.1002/ece3.398
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发表时间：
2012-12
影响因子：
2.6
通讯作者：
Johnsen, Arild
中科院分区：
文献类型：
--
作者：
Hogner, Silje;Laskemoen, Terje;Lifjeld, Jan T.;Porkert, Jiri;Kleven, Oddmund;Albayrak, Tamer;Kabasakal, Bekir;Johnsen, Arild
线粒体DNA在物种内序列变异小，在物种间序列差异大，这使其成为系统发育推断和DNA条形码的有用标记。先前对红尾莺（Phoenicurus phoenicurus）的研究揭示了两个非常不同的mtDNA单倍型群（5%K2P距离）。这种差异与许多姐妹物种之间的差异相当;然而，这两个单倍型群在今天的欧洲共存和杂交。在这里，我们描述了这些谱系的地理分布模式，并测试了mtDNA如此高的多样性是如何进化的假设。我们没有发现线粒体假基因的证据证实这两种单倍型都是线粒体起源的。在测试可能的生殖障碍时，我们没有发现谱系特异性交配的证据，精子形态也没有差异，这表明它们不是神秘物种的例子，也不太可能反映物种形成的早期阶段。一个基因树的基础上的一个短片段的细胞色素c氧化酶亚基1从常见的红尾燕和其他10个Phoenicurus物种，没有表现出渗入任何现存的同源。然而，不能排除从灭绝的同源基因渗入。来自两个核内含子的序列没有显示出类似的分化成两个不同的组。不匹配的分布表明，血统经历了类似的人口变化。总之，这些结果证实了差异很大的线粒体谱系可以在生物物种中共存。同域性mtDNA分歧在鸟类中相对罕见，但它们发生的事实反对在物种划分中使用阈值mtDNA分歧。
Mitochondrial DNA usually shows low sequence variation within and high sequence divergence among species, which makes it a useful marker for phylogenetic inference and DNA barcoding. A previous study on the common redstart (Phoenicurus phoenicurus) revealed two very different mtDNA haplogroups (5% K2P distance). This divergence is comparable to that among many sister species; however, both haplogroups coexist and interbreed in Europe today. Herein, we describe the phylogeographic pattern of these lineages and test hypotheses for how such high diversity in mtDNA has evolved. We found no evidence for mitochondrial pseudogenes confirming that both haplotypes are of mitochondrial origin. When testing for possible reproductive barriers, we found no evidence for lineage-specific assortative mating and no difference in sperm morphology, indicating that they are not examples of cryptic species, nor likely to reflect the early stages of speciation. A gene tree based on a short fragment of cytochrome c oxidase subunit 1 from the common redstart and 10 other Phoenicurus species, showed no introgression from any of the extant congenerics. However, introgression from an extinct congeneric cannot be excluded. Sequences from two nuclear introns did not show a similar differentiation into two distinct groups. Mismatch distributions indicated that the lineages have undergone similar demographic changes. Taken together, these results confirm that deeply divergent mitochondrial lineages can coexist in biological species. Sympatric mtDNA divergences are relatively rare in birds, but the fact that they occur argues against the use of threshold mtDNA divergences in species delineation.
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DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
9.8
作者：
Hebert PD;Stoeckle MY;Zemlak TS;Francis CM
通讯作者：
Francis CM
影响因子：
3.4
作者：
Haine, Eleanor R.;Martin, Joanne;Cook, James M.
通讯作者：
Cook, James M.
影响因子：
3.7
作者：
Kerr KC;Lijtmaer DA;Barreira AS;Hebert PD;Tubaro PL
通讯作者：
Tubaro PL
影响因子：
2.8
作者：
Kerr KC;Birks SM;Kalyakin MV;Red'kin YA;Koblik EA;Hebert PD
通讯作者：
Hebert PD