Effects of eicosapentaenoic acid and docosahexaenoic acid on responses of LPS-stimulated intestinal B lymphocytes from broiler chickens studied in vitro
Effects of eicosapentaenoic acid and docosahexaenoic acid on responses of LPS-stimulated intestinal B lymphocytes from broiler chickens studied in vitro
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二十碳五烯酸和二十二碳六烯酸对体外研究的肉鸡 LPS 刺激肠道 B 淋巴细胞反应的影响
DOI：
10.1007/s00217-011-1554-1
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发表时间：
2011-08
影响因子：
3.3
通讯作者：
Yao, Jun Hu
中科院分区：
文献类型：
--
作者：
Wang, Yi Bing;Yang, Xiao Jun;Qin, Ding Kui;Feng, Yan;Guo, Yu Ming;Yao, Jun Hu
采用肉鸡体外培养细胞，研究了不同比例和浓度的二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对肠道B淋巴细胞增殖、IgA分泌和CD5+CD79a表达的影响。用四甲基偶氮唑蓝(3-(4，5-二甲基噻唑-2-基)-2，5-二苯基四氮唑溴化法)检测细胞增殖，选择差异显著的组用ELISA试剂盒检测IgA。根据免疫球蛋白A分泌的显著差异，用荧光抗体染色和流式细胞术分析CD5和CD79a的表达。结果表明，EPA和DHA均能抑制脂多糖(LPS)刺激的B淋巴细胞体外增殖。EPA和DHA显著抑制内毒素刺激的B细胞分泌Ig A的能力，在20μg/m L时为1：1，在20和40μg/L时为2：1(P<；P<0.05)。在20μg/mLEPA/DHA比值为2：1时，CD5和CD79a在内毒素刺激的B细胞上表达最高(P<0.01)，提示炎症反应可能是通过增加CD5和CD79a的表达，抑制B细胞分泌Ig A而下调的。
Effects of different ratios and concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on intestinal B lymphocyte proliferation, IgA secretion and expression of CD5 + CD79a were studied in vitro using cells isolated from broilers. Proliferation was determined by the 3-(4,5- dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay, and those groups with significant difference were selected to detect the IgA using ELISA kit. According to the significant difference of IgA secretion, the expression of CD5 and CD79a was analyzed by fluorescent antibody staining and flow cytometry. The results showed that EPA and DHA inhibited the in vitro proliferation of lipopolysaccharide (LPS)-stimulated B lymphocytes. EPA and DHA significantly suppressed the ability of LPS-stimulated B cells to secrete IgA with ratios of 1:1 at 20 μg/mL and 2:1 at 20 and 40 μg/mL (P< 0.05). In addition, CD5 and CD79a were maximally expressed on LPS-stimulated B cells when the EPA/DHA ratio was 2:1 at 20 μg/mL (P< 0.01), suggesting that the inflammatory reaction may be downregulated by the increasing expression of CD5 and CD79a and inhibitive secretion of IgA by B cell.
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影响因子：
13.6
作者：
Kim, Wooki;Khan, Naim A.;McMurray, David N.;Prior, Ian A.;Wang, Naisyin;Chapkin, Robert S.
通讯作者：
Chapkin, Robert S.
影响因子：
4.2
作者：
Beli, Eleni;Li, Maoxiang;Pestka, James J.
通讯作者：
Pestka, James J.
影响因子：
2.1
作者：
P. Kankaanpää;Seppo Salminen;E. Isolauri;Y. Lee
通讯作者：
P. Kankaanpää;Seppo Salminen;E. Isolauri;Y. Lee
影响因子：
24.5
作者：
DAVIES, MDJ;PARROTT, DMV
通讯作者：
PARROTT, DMV
影响因子：
2
作者：
A. Hekmatdoust;Mirshafiei Abas;Jazayeri Abou Alghasem;M. Eshraghian;M. Feyzabadi;R. Sedaghat;K. Jakobsen
通讯作者：
A. Hekmatdoust;Mirshafiei Abas;Jazayeri Abou Alghasem;M. Eshraghian;M. Feyzabadi;R. Sedaghat;K. Jakobsen