Accumulation of tissue factor into developing thrombi in vivo is dependent upon microparticle P-selectin glycoprotein ligand 1 and platelet P-selectin.
Accumulation of tissue factor into developing thrombi in vivo is dependent upon microparticle P-selectin glycoprotein ligand 1 and platelet P-selectin.
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DOI：
10.1084/jem.20021868
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发表时间：
2003-06-02
期刊：
影响因子：
--
通讯作者：
Furie B
中科院分区：
文献类型：
--
作者：
Falati S;Liu Q;Gross P;Merrill-Skoloff G;Chou J;Vandendries E;Celi A;Croce K;Furie BC;Furie B
使用激光诱导的内皮损伤模型，我们通过实时体内显微镜检查了野生型和基因改造小鼠的微循环中的血栓形成，以分析包括血管壁、流动血液的存在和抗凝剂的缺乏的系统中的这一复杂的生理过程。我们观察了野生型小鼠小动脉中正在形成的血栓中血小板定位后的 P-选择素表达、组织因子积累和纤维蛋白生成。然而，缺乏 P-选择素糖蛋白配体 1 (PSGL-1) 或 P-选择素的小鼠，或注入阻断性 P-选择素抗体的野生型小鼠，会形成含有最少组织因子和纤维蛋白的血小板血栓。为了探索组织因子向正在形成的血栓的传递，我们在人贫血小板血浆中鉴定了单核细胞衍生的微粒，这些微粒表达组织因子、PSGL-1和CD14。将荧光标记的小鼠微粒注入位于正在形成的血栓内的受体小鼠体内，表明体内启动血液凝固的一种途径涉及含有组织因子和 PSGL-1 的微粒在表达 P-选择素的血小板血栓中的积累。这些单核细胞衍生的微粒通过血小板 P-选择素和微粒 PSGL-1 介导的相互作用与活化的血小板结合。我们认为，PSGL-1 除了在白细胞运输中发挥已知作用外，还在血液凝固中发挥作用。
Using a laser-induced endothelial injury model, we examined thrombus formation in the microcirculation of wild-type and genetically altered mice by real-time in vivo microscopy to analyze this complex physiologic process in a system that includes the vessel wall, the presence of flowing blood, and the absence of anticoagulants. We observe P-selectin expression, tissue factor accumulation, and fibrin generation after platelet localization in the developing thrombus in arterioles of wild-type mice. However, mice lacking P-selectin glycoprotein ligand 1 (PSGL-1) or P-selectin, or wild-type mice infused with blocking P-selectin antibodies, developed platelet thrombi containing minimal tissue factor and fibrin. To explore the delivery of tissue factor into a developing thrombus, we identified monocyte-derived microparticles in human platelet–poor plasma that express tissue factor, PSGL-1, and CD14. Fluorescently labeled mouse microparticles infused into a recipient mouse localized within the developing thrombus, indicating that one pathway for the initiation of blood coagulation in vivo involves the accumulation of tissue factor– and PSGL-1–containing microparticles in the platelet thrombus expressing P-selectin. These monocyte-derived microparticles bind to activated platelets in an interaction mediated by platelet P-selectin and microparticle PSGL-1. We propose that PSGL-1 plays a role in blood coagulation in addition to its known role in leukocyte trafficking.
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DOI：
10.1073/pnas.91.19.8767
发表时间：
1994-09-13
影响因子：
11.1
作者：
CELI, A;PELLEGRINI, G;FURIE, B
通讯作者：
FURIE, B
DOI：
10.1083/jcb.118.2.445
发表时间：
1992-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Moore KL;Stults NL;Diaz S;Smith DF;Cummings RD;Varki A;McEver RP
通讯作者：
McEver RP
影响因子：
15.9
作者：
LORENZET, R;NIEMETZ, J;BROEKMAN, MJ
通讯作者：
BROEKMAN, MJ
影响因子：
64.5
作者：
MAYADAS, TN;JOHNSON, RC;WAGNER, DD
通讯作者：
WAGNER, DD
影响因子：
3.6
作者：
KUDRYK, B;ROHOZA, A;WIEBE, ME
通讯作者：
WIEBE, ME