Male-female communication triggers calcium signatures during fertilization in Arabidopsis.
Male-female communication triggers calcium signatures during fertilization in Arabidopsis.
复制标题
DOI：
10.1038/ncomms5645
复制
发表时间：
2014-08-22
影响因子：
16.6
通讯作者：
Grossmann, Guido
中科院分区：
文献类型：
--
作者：
Denninger, Philipp;Bleckmann, Andrea;Lausser, Andreas;Vogler, Frank;Ott, Thomas;Ehrhardt, David W.;Frommer, Wolf B.;Sprunck, Stefanie;Dresselhaus, Thomas;Grossmann, Guido
细胞间通讯和相互作用在受精过程中至关重要，并触发细胞内游离钙（[Ca2+]cyto）作为卵子激活和动物卵母细胞多精受精阻滞的关键信号。有花植物的受精作用更为复杂，包括花粉管与邻近助细胞的卵细胞相互作用，最终释放两个精细胞，并分别与卵细胞和中央细胞融合。在这里，我们报告的发生和[Ca2+]细胞信号在整个双受精过程中的作用在拟南芥。[Ca2[+]助细胞与花粉管顶端接触后开始振荡。在卵细胞和中央细胞中，一个短的[Ca2+]细胞瞬变与花粉管爆裂和精子细胞的到达有关。仅在卵细胞中的第二个扩展的[Ca2+]cyto瞬时与成功受精相关。因此，每种参与双受精的雌性细胞类型显示出特征性的[Ca2+]cyto签名，其时间和行为与哺乳动物中报道的[Ca2+]cyto波不同。 受精涉及物种特异性的相互作用和最终的两个配子细胞的融合，钙信号在这个过程中起着关键作用。在这里，作者监测了开花植物受精过程中细胞内钙的变化，并指出了与该过程的特定步骤相关的特定特征。
Cell–cell communication and interaction is critical during fertilization and triggers free cytosolic calcium ([Ca2+]cyto) as a key signal for egg activation and a polyspermy block in animal oocytes. Fertilization in flowering plants is more complex, involving interaction of a pollen tube with egg adjoining synergid cells, culminating in release of two sperm cells and their fusion with the egg and central cell, respectively. Here, we report the occurrence and role of [Ca2+]cyto signals during the entire double fertilization process in Arabidopsis. [Ca2+]cyto oscillations are initiated in synergid cells after physical contact with the pollen tube apex. In egg and central cells, a short [Ca2+]cyto transient is associated with pollen tube burst and sperm cell arrival. A second extended [Ca2+]cyto transient solely in the egg cell is correlated with successful fertilization. Thus, each female cell type involved in double fertilization displays a characteristic [Ca2+]cyto signature differing by timing and behaviour from [Ca2+]cyto waves reported in mammals. Fertilization involves species-specific interaction and eventually fusion of two gametic cells, and calcium signalling plays a key role in this process. Here, the authors monitor the changes in intracellular calcium that take place during fertilization of flowering plants and point to specific signatures associated with specific steps of the process.
登录
查看更多内容
DOI：
10.1126/science.1244454
发表时间：
2014-01-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Haruta M;Sabat G;Stecker K;Minkoff BB;Sussman MR
通讯作者：
Sussman MR
影响因子：
9.2
作者：
Beale, Kristin M.;Leydon, Alexander R.;Johnson, Mark A.
通讯作者：
Johnson, Mark A.
影响因子：
56.9
作者：
FAURE, JE;DIGONNET, C;DUMAS, C
通讯作者：
DUMAS, C
影响因子：
11.6
作者：
Capron, Arnaud;Gourgues, Mathieu;Sundaresan, Venkatesan
通讯作者：
Sundaresan, Venkatesan
影响因子：
9.8
作者：
Boisson-Dernier A;Lituiev DS;Nestorova A;Franck CM;Thirugnanarajah S;Grossniklaus U
通讯作者：
Grossniklaus U