In vivo voltammetric monitoring of catecholamine release in subterritories of the nucleus accumbens shell.
In vivo voltammetric monitoring of catecholamine release in subterritories of the nucleus accumbens shell.
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DOI：
10.1016/j.neuroscience.2010.04.076
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发表时间：
2010-08-11
期刊：
影响因子：
3.3
通讯作者：
Wightman, R. M.
中科院分区：
文献类型：
--
作者：
Park, J.;Aragona, B. J.;Kile, B. M.;Carelli, R. M.;Wightman, R. M.
关键词：
在碳纤维微电极上的快速扫描循环伏安法（FSCV）已被用来证明，在动机行为过程中，儿茶酚胺浓度的亚秒级变化发生在神经核（NAc）壳内，这些波动已被归因于快速多巴胺信号。然而，FSCV不能区分多巴胺和去甲肾上腺素，NAc壳的尾部区域接受去甲肾上腺素能投射。因此，在本研究中，我们研究了去甲肾上腺素在何种程度上有助于儿茶酚胺释放内的喙和尾部的NAC壳。组织含量的分析表明，多巴胺是主要的儿茶酚胺检测在喙NAC壳，而多巴胺和去甲肾上腺素被发现在尾部次区域。为了检查可释放的儿茶酚胺，电刺激被用来唤起释放在麻醉大鼠的内侧前脑束，一个途径含有多巴胺能和去甲肾上腺素能投射到NAc，或腹侧被盖区/黑质，多巴胺能投射的起源刺激。通过对不同药物的反应来区分儿茶酚胺。多巴胺自身受体阻滞剂，雷氯必利，以及单胺和多巴胺转运体阻滞剂，可卡因和GBR 12909，增加诱发的儿茶酚胺溢出在吻侧和尾侧NAc壳。去甲肾上腺素自身受体阻滞剂育亨宾和去甲肾上腺素转运体阻滞剂地昔帕明增加了尾侧NAc壳中的儿茶酚胺溢出，而没有显著改变喙侧NAc壳中的诱发反应。因此，神经化学和药理学结果表明，去甲肾上腺素信号仅限于尾侧部分的NAc壳。在雷氯必利和可卡因或雷氯必利和GBR 12909后，在吻侧壳内观察到稳健的儿茶酚胺瞬变，但这些在尾侧NAc壳中远不明显，并且在育亨宾和地昔帕明后未发生。两者合计，数据表明，在吻侧NAc壳检测到的FSCV的儿茶酚胺信号是由于多巴胺传输的变化。
Fast-scan cyclic voltammetry (FSCV) at carbon-fiber microelectrodes has been used to demonstrate that sub-second changes in catecholamine concentration occur within the nucleus accumbens (NAc) shell during motivated behaviors, and these fluctuations have been attributed to rapid dopamine signaling. However, FSCV cannot distinguish between dopamine and norepinephrine, and caudal regions of the NAc shell receive noradrenergic projections. Therefore, in the present study, we examined the degree to which norepinephrine contributes to catecholamine release within rostral and caudal portion of NAc shell. Analysis of tissue content revealed that dopamine was the major catecholamine detectable in the rostral NAc shell, whereas both dopamine and norepinephrine were found in the caudal subregion. To examine releasable catecholamines, electrical stimulation was used to evoke release in anesthetized rats with either stimulation of the medial forebrain bundle, a pathway containing both dopaminergic and noradrenergic projections to the NAc, or the ventral tegmental area/substantia nigra, the origin of dopaminergic projections. The catecholamines were distinguished by their responses to different pharmacological agents. The dopamine autoreceptor blocker, raclopride, as well as the monoamine and dopamine transporter blockers, cocaine and GBR 12909, increased evoked catecholamine overflow in both the rostral and caudal NAc shell. The norepinephrine autoreceptor blocker, yohimbine, and the norepinephrine transporter blocker, desipramine, increased catecholamine overflow in the caudal NAc shell without significant alteration of evoked responses in the rostral NAc shell. Thus, the neurochemical and pharmacological results show that norepinephrine signaling is restricted to caudal portions of the NAc shell. Following raclopride and cocaine or raclopride and GBR 12909, robust catecholamine transients were observed within the rostral shell but these were far less apparent in the caudal NAc shell, and they did not occur following yohimbine and desipramine. Taken together, the data demonstrate that catecholamine signals in the rostral NAc shell detected by FSCV are due to change in dopamine transmission.
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影响因子：
3.3
作者：
Espana, R. A.;Roberts, D. C. S.;Jones, S. R.
通讯作者：
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2.3
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