Immunohistochemical characterisation of PCV2 associate lesions in lymphoid and non-lymphoid tissues of pigs with natural postweaning multisystemic wasting syndrome (PMWS).
Immunohistochemical characterisation of PCV2 associate lesions in lymphoid and non-lymphoid tissues of pigs with natural postweaning multisystemic wasting syndrome (PMWS).
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DOI：
10.1016/s0165-2427(03)00079-5
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发表时间：
2003-07-15
影响因子：
1.8
通讯作者：
Domingo M
中科院分区：
文献类型：
--
作者：
Chianini F;Majó N;Segalés J;Domínguez J;Domingo M
用免疫组织学方法研究了自然发生的断奶后多系统衰竭综合征（PMWS）病猪的淋巴、肾、肺和肝病变。从对猪生殖和呼吸道病毒以及Aujeszky病病毒呈血清阴性的农场中选择10头表现出PMWS急性临床体征的常规饲养猪。所有猪在ISH和IHC检测猪圆环病毒2型中均呈阳性。使用抗CD 3、CD 79 α、CD 45 RA（3C 3/9）、溶菌酶、SLA-II-DQ（BL 2 H5）和MAC 387的单克隆和多克隆抗体对PMWS病变中的细胞进行标记。与未感染对照组相比，最相关的变化是B和T淋巴细胞减少或丢失、巨噬细胞数量增加以及整个淋巴组织中抗原呈递细胞的部分丢失和重新分布。本研究中淋巴病变的特征强烈表明PMWS在受影响猪中具有免疫抑制作用。
The lymphoid, renal, pulmonary, and hepatic lesions of naturally occurring postweaning multisystemic wasting syndrome (PMWS) affected pigs have been studied by means of immunohistology. Ten conventionally reared pigs showing acute clinical signs of PMWS were selected from a farm on which animal were seronegative to porcine reproductive and respiratory virus and to Aujeszky’s disease virus. All pigs were positive in tests for porcine circovirus type 2 by ISH and IHC. Monoclonal and polyclonal antibodies to CD3, CD79α, CD45RA (3C3/9), lysozyme, SLA-II-DQ (BL2H5), and MAC387 were used to characterise cells in PMWS lesions. The most relevant changes were reduction or loss of B and T lymphocytes, increased numbers of macrophages, and partial loss and redistribution of antigen presenting cells throughout lymphoid tissues compared to uninfected controls. The characteristics of lymphoid lesions in the present study strongly suggest an immunosuppressive effect of PMWS in affected pigs.
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影响因子：
2.4
作者：
Harms, PA;Sorden, SD;Paul, PS
通讯作者：
Paul, PS
影响因子：
1.8
作者：
Chianini, F;Majó, N;Domingo, M
通讯作者：
Domingo, M
影响因子：
1.5
作者：
Sorden, SD;Harms, PA;Paul, PS
通讯作者：
Paul, PS
影响因子：
2.2
作者：
Carrasco, L;Segalés, J;Sierra, MA
通讯作者：
Sierra, MA
DOI：
10.1128/cdli.9.2.236-242.2002
发表时间：
2002-03-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Darwich, L;Segalés, J;Mateu, E
通讯作者：
Mateu, E