Plant Cadmium Resistance 2 (SaPCR2) Facilitates Cadmium Efflux in the Roots of Hyperaccumulator Sedum alfredii Hance.
Plant Cadmium Resistance 2 (SaPCR2) Facilitates Cadmium Efflux in the Roots of Hyperaccumulator Sedum alfredii Hance.
复制标题
镉抗性 2 (SaPCR2) 促进超富集植物景天 (Sedum alfredii Hance) 根部的镉流出
DOI：
10.3389/fpls.2020.568887
复制
发表时间：
2020
影响因子：
5.6
通讯作者：
Lu L
中科院分区：
文献类型：
--
作者：
Lin J;Gao X;Zhao J;Zhang J;Chen S;Lu L
金丝桃是植物修复的首选材料。东南景天（Sedum alfredii Hance）是我国的一种镉超富集植物，但其解毒机制尚未完全阐明。在本研究中，我们从超积累生态型（HE）的水稻中克隆了一个属于植物镉抗性（PCR）家族的基因，命名为SaPCR 2。alfredii。序列分析表明，SaPCR 2含有一个在PCR家族中高度保守的富含半胱氨酸的结构域，在镉的解毒过程中发挥重要作用。相对定量结果表明，SaPCR 2在HE S根中高表达。但对紫茎泽兰的SaPCR 2表达没有显著影响。相反，SaPCR 2在其非超积累生态型（NHE）植物中的表达水平非常低。SaPCR 2在烟草叶片和酵母中的亚细胞定位结果表明，SaPCR 2定位于质膜上，在Zn/Cd敏感酵母Δ zrc 1中表达SaPCR 2蛋白可显著提高其对Cd胁迫的耐受性，降低细胞内Cd含量。SaPCR 2在拟南芥和NHE S. alfredii显著降低了根中Cd含量，但对地上部Cd含量无显著影响。这些结果表明，SaPCR 2在植物中的过量表达提供了一个途径，镉渗漏出的根细胞和保护根细胞免受镉胁迫的植物毒性。据我们所知，这是第一次研究超积累植物S. alfredii。
Hyperaccumulators are the preferred materials for phytoremediation. Sedum alfredii Hance is a cadmium (Cd) hyperaccumulator plant in China, although its detoxification mechanism remains unresolved. In our study, we cloned a gene belonging to the plant cadmium resistance (PCR) family, named SaPCR2, from the hyperaccumulating ecotype (HE) of S. alfredii. Sequence analysis indicated that SaPCR2 contained a cysteine-rich domain highly conserved in the PCR family and played an important role in Cd detoxification. Based on the relative quantitative results, SaPCR2 was highly expressed in the roots of HE S. alfredii, but not the shoots and Cd exposure did not significantly affect SaPCR2 expression. In contrast, the expression level of SaPCR2 was very low in plants of its non-hyperaccumulating ecotype (NHE). The subcellular localization of SaPCR2 in tobacco leaves and yeasts showed that SaPCR2 was localized on the plasma membrane and the expression of the SaPCR2 protein in a Zn/Cd-sensitive yeast Δzrc1 significantly increased its tolerance to Cd stress by decreasing the Cd content in cells. Heterologous expression of SaPCR2 in plants of both Arabidopsis thaliana and NHE S. alfredii significantly reduced the Cd levels in the roots, but not in the shoots. These results suggest that the overexpression of SaPCR2 in plants provides a route for Cd leak out of the root cells and protects the root cells against phytotoxicity of Cd stress. To the best of our knowledge, this is the first study of transporter-mediated root efflux of Cd in hyperaccumulator S. alfredii.
登录
查看更多内容
影响因子：
14.8
作者：
Gietz, R. Daniel;Schiestl, Robert H.
通讯作者：
Schiestl, Robert H.
影响因子：
5.7
作者：
Chao, Yue-en;Zhang, Min;Islam, Ejazul
通讯作者：
Islam, Ejazul
影响因子：
9.4
作者：
Liu, Huan;Zhao, Haixia;Xu, Wenzhong
通讯作者：
Xu, Wenzhong
景天超富集生态型中镉从根到茎的易位增强
影响因子：
6.9
作者：
Lu, Ling-li;Tian, Sheng-ke;Yang, Xiao-e;Wang, Xiao-chang;Brown, Patrick;Li, Ting-qiang;He, Zhen-li
通讯作者：
He, Zhen-li
重金属超富集植物景天镉胁迫反应的转录组分析
影响因子：
3.7
作者：
Gao J;Sun L;Yang X;Liu JX
通讯作者：
Liu JX