A class of independently evolved transcriptional repressors in plant RNA viruses facilitates viral infection and vector feeding.
A class of independently evolved transcriptional repressors in plant RNA viruses facilitates viral infection and vector feeding.
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DOI：
10.1073/pnas.2016673118
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发表时间：
2021-03-16
影响因子：
11.1
通讯作者：
Sun Z
中科院分区：
文献类型：
--
作者：
Li L;Zhang H;Chen C;Huang H;Tan X;Wei Z;Li J;Yan F;Zhang C;Chen J;Sun Z
为了完成侵染循环，植物病毒通常利用各种致病因子来抑制宿主的抗病毒防御。揭示不同病毒之间保守的致病机制对于发展广谱疾病控制至关重要。在这里，我们表明，一类独立进化的转录抑制因子广泛存在于不同的植物病毒。尽管这些转录抑制因子的序列不同，进化起源也不同，但它们都通过抑制OsMYC转录因子的转录激活，解离OsMED25-OsMYC复合物，并与OsJAZ协同作用提高转录抑制活性来调节JA信号传导。这促进了病毒感染并增强了载体喂养。我们的研究结果揭示了植物病毒中转录抑制因子的关键功能，并揭示了病毒致病性和载体传播的一般机制。植物病毒采用不同的毒力策略来实现成功的感染，但是很少有已知的广泛不同的植物病毒使用的病毒致病性和传播的一般策略。在这里，我们报告了一类独立进化的毒力因子在不同的植物RNA病毒，具有积极的转录抑制活性。斐济病毒属（Fijivirus）、细条病毒属（Tenuivirus）和细胞弹状病毒属（Cytorhabdovirus）中的水稻病毒都具有转录阻遏物，其在植物中与茉莉酸（JA）信号传导的关键组分，即介体亚基OsMED25、OsJAZ蛋白和OsMYC转录因子相互作用。这些转录抑制因子可以直接解离OsMED25-OsMYC复合物，抑制OsMYC的转录激活，然后与OsJAZ蛋白联合收割机结合，以有利于病毒感染的方式协同减弱JA途径。同时，这些转录抑制因子通过抑制JA信号传导有效地增强了病毒昆虫载体的摄食。我们的研究结果揭示了一个共同的策略，在不相关的植物病毒中，病毒转录抑制因子劫持和抑制JA途径，有利于病毒的致病性和载体传播。
To accomplish the infection cycle, plant viruses usually employ various pathogenic factors to inhibit host antiviral defense. Revealing pathogenic mechanisms conserved among different viruses is essential for developing broad-spectrum disease control. Here, we show that a class of independently evolved transcriptional repressors is widely present in different plant viruses. Despite the diverse sequences and different evolutionary origins, these transcriptional repressors all modulate JA signaling by inhibiting the transcriptional activation of OsMYC transcription factors, disassociating the OsMED25-OsMYC complex, and cooperating with OsJAZ to improve their transcriptional repression activity. This facilitates viral infection and enhances vector feeding. Our findings reveal key functions of the transcriptional repressors in plant viruses and shed light on the general mechanism of viral pathogenicity and vector transmission. Plant viruses employ diverse virulence strategies to achieve successful infection, but there are few known general strategies of viral pathogenicity and transmission used by widely different plant viruses. Here, we report a class of independently evolved virulence factors in different plant RNA viruses which possess active transcriptional repressor activity. Rice viruses in the genera Fijivirus, Tenuivirus, and Cytorhabdovirus all have transcriptional repressors that interact in plants with the key components of jasmonic acid (JA) signaling, namely mediator subunit OsMED25, OsJAZ proteins, and OsMYC transcription factors. These transcriptional repressors can directly disassociate the OsMED25-OsMYC complex, inhibit the transcriptional activation of OsMYC, and then combine with OsJAZ proteins to cooperatively attenuate the JA pathway in a way that benefits viral infection. At the same time, these transcriptional repressors efficiently enhanced feeding by the virus insect vectors by repressing JA signaling. Our findings reveal a common strategy in unrelated plant viruses in which viral transcriptional repressors hijack and repress the JA pathway in favor of both viral pathogenicity and vector transmission.
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