Genome-wide analysis of valine-glutamine motif-containing proteins related to abiotic stress response in cucumber (Cucumis sativus L.).
Genome-wide analysis of valine-glutamine motif-containing proteins related to abiotic stress response in cucumber (Cucumis sativus L.).
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DOI：
10.1186/s12870-021-03242-9
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发表时间：
2021-10-25
影响因子：
5.3
通讯作者：
Gan Z
中科院分区：
文献类型：
--
作者：
Shan N;Xiang Z;Sun J;Zhu Q;Xiao Y;Wang P;Chen X;Zhou Q;Gan Z
黄瓜(Cucumis sativus L.)是一种重要的经济作物，易受各种非生物胁迫的影响。缬氨酸-谷氨酰胺(VQ)基序蛋白是植物特有的蛋白质，具有保守的FxxhVQxhTG氨基酸序列，调节植物的生长发育。然而，人们对黄瓜中VQ蛋白的功能知之甚少。本研究通过全基因组分析对黄瓜中的32个CsVQ蛋白进行了鉴定，它们都含有一个保守的基序和10个变异。系统进化树分析表明，这些CsVQ蛋白与拟南芥、甜瓜和水稻的CsVQ蛋白进行了比较，可分为9个类群。CsVQ基因分布在7条染色体上。这些基因中的大多数被预测定位在细胞核中。此外，在CsVQ基因的启动子中还观察到了顺式元件对不同胁迫和激素的响应。提出了一个与WRKY转录因子相互作用的CsVQ蛋白网络(CsWRKYs)。此外，CsVQ基因的转录产物具有时空特异性，受非生物逆境诱导。CsVQ4、CsVQ6、CsVQ16-2、CsVQ19、CsVQ24、CsVQ30、CsVQ32、CsVQ33和CsVQ34在器官和组织中表达水平较高，并能对多种激素和不同的胁迫做出反应，表明这些基因参与了对刺激的反应。综上所述，我们的研究结果揭示了新的黄瓜抗VQ基因资源，并提供了CsVQ基因及其编码蛋白的关键信息，为黄瓜抗VQ基因育种提供了重要的遗传基础。网上版载有补充材料，可在10.1186/s12870-021-03242-9查阅。
Cucumber (Cucumis sativus L.) is one of the most important economic crops and is susceptible to various abiotic stresses. The valine-glutamine (VQ) motif-containing proteins are plant-specific proteins with a conserved “FxxhVQxhTG” amino acid sequence that regulates plant growth and development. However, little is known about the function of VQ proteins in cucumber. In this study, a total of 32 CsVQ proteins from cucumber were confirmed and characterized using comprehensive genome-wide analysis, and they all contain a conserved motif with 10 variations. Phylogenetic tree analysis revealed that these CsVQ proteins were classified into nine groups by comparing the CsVQ proteins with those of Arabidopsis thaliana, melon and rice. CsVQ genes were distributed on seven chromosomes. Most of these genes were predicted to be localized in the nucleus. In addition, cis-elements in response to different stresses and hormones were observed in the promoters of the CsVQ genes. A network of CsVQ proteins interacting with WRKY transcription factors (CsWRKYs) was proposed. Moreover, the transcripts of CsVQ gene were spatio-temporal specific and were induced by abiotic adversities. CsVQ4, CsVQ6, CsVQ16–2, CsVQ19, CsVQ24, CsVQ30, CsVQ32, CsVQ33, and CsVQ34 were expressed in the range of organs and tissues at higher levels and could respond to multiple hormones and different stresses, indicating that these genes were involved in the response to stimuli. Together, our results reveal novel VQ resistance gene resources, and provide critical information on CsVQ genes and their encoded proteins, which supplies important genetic basis for VQ resistance breeding of cucumber plants. The online version contains supplementary material available at 10.1186/s12870-021-03242-9.
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影响因子：
7.2
作者：
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通讯作者：
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影响因子：
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