Functional stacking of three resistance genes against Phytophthora infestans in potato.
Functional stacking of three resistance genes against Phytophthora infestans in potato.
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DOI：
10.1007/s11248-011-9510-1
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发表时间：
2012-02
影响因子：
3
通讯作者：
Jacobsen E
中科院分区：
文献类型：
--
作者：
Zhu S;Li Y;Vossen JH;Visser RG;Jacobsen E
广谱抗性(R)基因的功能堆积可能是一种更持久的抗病的有效策略，例如，对马铃薯晚疫病菌(Phytophthora infestans，PI)的抗性。为此，我们选择了3个广谱马铃薯R基因(RPI)：RPI-sto1(茄子)、RPI-vnt1.1(茄子)和RPI-blb3(球茎茄子)，构建成一个双元载体pBINPLUS，转化感病品种Desiree。在550株卡那霉素抗性再生植株中，有28株进一步进行了基因特异性PCRS分析。所有再生植株均为nptII基因阳性，其中23株含有3个RPI基因，称为三重RPI基因转化。叶片离体法和无毒基因农业渗入试验表明，23个三重RPI基因转化子对供试菌株均表现抗性，并表现出抗性，并带有3个AVR效应基因，表明3个RPI基因均有功能堆积。结论是，与待堆叠的R基因相对应的AVR基因必须是可用的，以便检测每个堆叠组件的功能。没有发现沉默的迹象或任何其他影响插入的RPI基因功能的负面影响。不出所料，这23个三重RPI基因转化子的抗谱是3个单独RPI基因的抗谱之和。这是通过基因转化同时驯化三个自然R基因对抗单一疾病的第一个一步方法的例子。本文的在线版本(doi：10.1007/s112480119510-1)包含补充材料，授权用户可以使用。
Functional stacking of broad spectrum resistance (R) genes could potentially be an effective strategy for more durable disease resistance, for example, to potato late blight caused by Phytophthora infestans (Pi). For this reason, three broad spectrum potato R genes (Rpi), Rpi-sto1 (Solanum stoloniferum), Rpi-vnt1.1 (S. venturii) and Rpi-blb3 (S. bulbocastanum) were selected, combined into a single binary vector pBINPLUS and transformed into the susceptible cultivar Desiree. Among the 550 kanamycin resistant regenerants, 28 were further investigated by gene specific PCRs. All regenerants were positive for the nptII gene and 23 of them contained the three Rpi genes, referred to as triple Rpi gene transformants. Detached leaf assay and agro-infiltration of avirulence (Avr) genes showed that the 23 triple Rpi gene transformants were resistant to the selected isolates and showed HR with the three Avr effectors indicating functional stacking of all the three Rpi genes. It is concluded that Avr genes, corresponding to the R genes to be stacked, must be available in order to assay for functionality of each stack component. No indications were found for silencing or any other negative effects affecting the function of the inserted Rpi genes. The resistance spectrum of these 23 triple Rpi gene transformants was, as expected, a sum of the spectra from the three individual Rpi genes. This is the first example of a one-step approach for the simultaneous domestication of three natural R genes against a single disease by genetic transformation. The online version of this article (doi:10.1007/s11248-011-9510-1) contains supplementary material, which is available to authorized users.
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影响因子：
7.2
作者：
Bendahmane, A;Querci, M;Baulcombe, DC
通讯作者：
Baulcombe, DC
影响因子：
1.9
作者：
BLACK, W.;MASTENBROEK, C.;PETERSON, L. C.
通讯作者：
PETERSON, L. C.
影响因子：
7.2
作者：
Huang, SW;van der Vossen, EAG;Visser, RGF
通讯作者：
Visser, RGF
影响因子：
7.2
作者：
Ballvora, A;Ercolano, MR;Gebhardt, C
通讯作者：
Gebhardt, C
影响因子：
1.5
作者：
Andreu, Adriana B.;Caldiz, Daniel O.;Forbes, Gregory A.
通讯作者：
Forbes, Gregory A.