Segmental allergen challenge enhances chitinase activity and levels of CCL18 in mild atopic asthma.
Segmental allergen challenge enhances chitinase activity and levels of CCL18 in mild atopic asthma.
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DOI：
10.1111/cea.12032
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发表时间：
2013-02
期刊：
影响因子：
--
通讯作者：
Jarjour NN
中科院分区：
文献类型：
--
作者：
Gavala ML;Kelly EA;Esnault S;Kukreja S;Evans MD;Bertics PJ;Chupp GL;Jarjour NN
过敏性气道炎症导致气道重塑，而气道重塑与哮喘的阻塞和发病率增加有关。然而，过敏原促进人类呼吸道重塑的机制尚未完全确定。CCL18、壳三糖苷酶(CHIT1)和YKL-40在肺部很容易被检测到，并有助于其他纤维化疾病的重塑，但它们在过敏性哮喘中的作用尚不清楚。我们假设CCL18、YKL-40和CHIT1的生物活性在节段性过敏原攻击后在过敏性哮喘受试者中增强，并且与肺中纤维化和Th2相关介质的增加有关。检测哮喘患者基础状态和节段性变应原激发后48h支气管肺泡灌洗液(BAL)中CCL18、YKL-40蛋白水平和CHIT1生物活性，以及BAL细胞中CCL18、YKL-40和CHIT1mRNA水平。我们还研究了CCL18和YKL-40水平以及CHIT1活性与其他促纤维化因子和趋化因子水平之间的相关性，这些因子和趋化因子在过敏原攻击后上调。节段性变应原攻击后CHIT1活性、YKL-40和CCL18水平升高，这些水平与其他促纤维化因子、T细胞趋化因子和炎症细胞在变应原攻击后的水平相关。BAL细胞经变应原攻击后CCL18和YKL-40mRNA水平也升高。我们的结果提示，CCL18和YKL-40水平和CHIT1活性在变态反应性气道炎症中升高，从而可能参与哮喘的气道重塑。
Allergic airway inflammation contributes to the airway remodeling that has been linked to increased obstruction and morbidity in asthma. However, the mechanisms by which allergens contribute to airway remodeling in humans are not fully established. CCL18, chitotriosidase (CHIT1) and YKL-40 are readily detectable in the lungs and contribute to remodeling in other fibrotic diseases, but their involvement in allergic asthma is unclear. We hypothesized that CCL18, YKL-40, and CHIT1 bioactivity are enhanced in allergic asthma subjects after segmental allergen challenge and are related to increased profibrotic and Th2-associated mediators in the lungs. Levels of CCL18 and YKL-40 protein and CHIT1 bioactivity in bronchoalveolar lavage (BAL) fluid, as well as CCL18, YKL-40, and CHIT1 mRNA levels in BAL cells, were evaluated in patients with asthma at baseline and 48 h after segmental allergen challenge. We also examined the correlation between CCL18 and YKL-40 levels and CHIT1 activity with the levels of other profibrotic factors and chemokines previously shown to be up-regulated after allergen challenge. CHIT1 activity and YKL-40 and CCL18 levels were elevated after segmental allergen challenge and these levels correlated with those of other profibrotic factors, T cell chemokines, and inflammatory cells after allergen challenge. CCL18 and YKL-40 mRNA levels also increased in BAL cells after allergen challenge. Our results suggest that CCL18 and YKL-40 levels and CHIT1 activity are enhanced in allergic airway inflammation and thus may contribute to airway remodeling in asthma.
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影响因子：
9.6
作者：
Kim HB;Kim CK;Iijima K;Kobayashi T;Kita H
通讯作者：
Kita H
DOI：
10.1165/rcmb.2003-0078oc
发表时间：
2003-12-01
影响因子：
6.4
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1164/ajrccm.156.5.9703054
发表时间：
1997-11-01
影响因子：
24.7
作者：
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通讯作者：
Jarjour, NN
影响因子：
2.6
作者：
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通讯作者：
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