Protein microarray analysis in patients with asthma: elevation of the chemokine PARC/CCL18 in sputum.
Protein microarray analysis in patients with asthma: elevation of the chemokine PARC/CCL18 in sputum.
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作者：
Kim HB;Kim CK;Iijima K;Kobayashi T;Kita H
微阵列技术为深入了解哮喘的全球基因和蛋白质表达谱提供了新的机会。为了确定在哮喘呼吸道中产生的新因素，我们使用基于膜的人类细胞因子微阵列技术分析了哮喘患者的痰样本。诱导痰取自28例BA患者、20例非哮喘特应性对照组(AC)和38例非哮喘非特应性正常对照组(NC)。受试者的微阵列样本从9名BA受试者、3名AC受试者和6名NC受试者中随机选择。使用定制的人类细胞因子阵列(RayBiotech；RayBiotech；Norcross，GA)分析痰上清液，该阵列旨在同时分析79种特定的细胞因子。用双抗体夹心酶联免疫吸附试验(ELISA)检测肺组织生长调节癌基因(GRO)-α、嗜酸性粒细胞趋化因子-2(Eoaxin-2)、肺和活化调节趋化因子(PARC)/CCL18水平，放射免疫法测定嗜酸性粒细胞源性神经毒素(EDN)。基因芯片显示，BA组Gro-α、eoaxin-2和PARC的信号强度显著高于AC组和NC组(p=0.036、p=0.042和p=0.033)。酶联免疫吸附试验显示，BA患者的痰PARC蛋白水平显著高于AC和NC患者(p<；0.0001)。此外，PARC水平与痰嗜酸粒细胞百分率(r=0.570，p<；0.0001)、内源性神经营养因子(r=0.633，p<；0.0001)、活化后受调节的正常T细胞表达和分泌的细胞因子(r=0.440，p<；0.001)、白介素4(r=0.415，p<；0.01)和干扰素-γ(r=0.491，p<；0.001)水平显著相关。通过一种无偏见的筛查方法，趋化因子PARC在哮喘患者的痰标本中升高。PARC可能在哮喘气道嗜酸性炎症的发生发展中起重要作用。
Microarray technology offers a new opportunity to gain insight into global gene and protein expression profiles in asthma. To identify novel factors produced in the asthmatic airway, we analyzed sputum samples by using a membrane-based human cytokine microarray technology in patients with bronchial asthma (BA). Induced sputum was obtained from 28 BA subjects, 20 nonasthmatic atopic control (AC) subjects, and 38 nonasthmatic nonatopic normal control (NC) subjects. The microarray samples of subjects were randomly selected from nine BA subjects, three AC subjects, and six NC subjects. Sputum supernatants were analyzed using a custom human cytokine array (RayBio Custom Human Cytokine Array; RayBiotech; Norcross, GA) designed to analyze 79 specific cytokines simultaneously. The levels of growth-regulated oncogene (GRO)-α, eotaxin-2, and pulmonary and activation-regulated chemokine (PARC)/CCL18 were measured by sandwich enzyme-linked immunosorbent assays (ELISAs), and eosinophil-derived neurotoxin (EDN) was measured by radioimmunoassay. By microarray, the signal intensities for GRO-α, eotaxin-2, and PARC were significantly higher in BA subjects than in AC and NC subjects (p = 0.036, p = 0.042, and p = 0.033, respectively). By ELISA, the sputum PARC protein levels were significantly higher in BA subjects than in AC and NC subjects (p < 0.0001). Furthermore, PARC levels correlated significantly with sputum eosinophil percentages (r = 0.570, p < 0.0001) and the levels of EDN(r = 0.633, p < 0.0001), the regulated upon activation, normal T cell expressed and secreted cytokine (r = 0.440, p < 0.001), interleukin-4 (r = 0.415, p < 0.01), and interferon-γ (r = 0.491, p < 0.001). By a nonbiased screening approach, a chemokine, PARC, is elevated in sputum specimens from patients with asthma. PARC may play important roles in development of airway eosinophilic inflammation in asthma.
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影响因子：
14.2
作者：
Nomura, L;Gao, B;Leung, DYM
通讯作者：
Leung, DYM
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14.2
作者：
CLAMAN, DM;BOUSHEY, HA;FAHY, JV
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影响因子：
4.4
作者：
Guan, P;Burghes, AHM;McColl, SR
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影响因子：
3.3
作者：
Bisset, LR;Schmid-Grendelmeier, P
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Schmid-Grendelmeier, P
影响因子：
64.8
作者：
Adema, GJ;Hartgers, F;Figdor, CG
通讯作者：
Figdor, CG