Phenolic contents, antioxidant and anticholinesterase potentials of crude extract, subsequent fractions and crude saponins from Polygonum hydropiper L.
Phenolic contents, antioxidant and anticholinesterase potentials of crude extract, subsequent fractions and crude saponins from Polygonum hydropiper L.
复制标题
DOI：
10.1186/1472-6882-14-145
复制
发表时间：
2014-05-03
影响因子：
--
通讯作者：
Imran M
中科院分区：
文献类型：
--
作者：
Ayaz M;Junaid M;Ahmed J;Ullah F;Sadiq A;Ahmad S;Imran M
对辣蓼属植物蓼Polygonum hydropiper L. (P. Hydropiper）的酚含量、抗氧化剂、抗胆碱酯酶活性，试图使其在神经系统疾病中的使用合理化。研究了植物粗提物（Ph.Cr）、正己烷（Ph.Hex）、氯仿（Ph.Chf）、乙酸乙酯（Ph.EtAc）、正丁醇（Ph.Bt）、水溶液（Ph.Aq）和皂甙（Ph.Sp）对1，1-二苯基，2-苦肼基（DPPH）、2，2-连氮双[3-乙基苯并噻唑啉]-6-磺酸（ABTS）自由基的清除能力。此外，乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的抑制活性进行了使用Ellman的测定。此外，测定植物提取物的总酚含量，并以mg没食子酸当量/克干样品（mg GAE/g干重）表示。不同部位中，酚类物质含量较高的有Ph.Cr（90.82）、Ph.Chf（178.16）、Ph.EtAc（203.44）和Ph.Bt（153.61）。各组分清除DPPH自由基的活性均呈浓度依赖性，其中以乙酸乙酯71.33%（IC 50 15 μg/ml）、Bt 71.40%（IC 50 3 μg/ml）和Sp 71.40%（IC 50 35 μg/ml）的活性最强。结果表明：4种植物提取物对ABTS的清除率分别为：Ph.Bt（91.03%），Ph.EtAc（90.56%），Ph.Sp（90.84%），Ph.Aq（90.56%），IC 50 < 0.01 μg/ml。所有组分均显示出中等至高的AChE抑制活性，在1 mg/ml时，Ph.Cr，86.87%（IC 50 330 μg/ml），Ph.Hex，87.49%（IC 50 35 μ g/ml），Ph.Chf，84.76%（IC 50 55 μg/ml），Ph.Sp，87.58%（IC 50 108 μg/ml）和Ph.EtAc 79.95%（IC 50 310 μg/ml）。此外，对BChE抑制活性最强的是Ph.Hex 90.30%（IC 50 40 μg/ml）、Ph.Chf 85.94%（IC 50 215 μg/ml）、Ph.Aq 87.62%（IC 50 3 μg/ml）和Ph.EtAc 81.01%（IC 50 395 μg/ml）。本研究首次对水辣蓼提取物进行了酚类物质含量测定、粗皂苷分离、抗氧化和抗胆碱酯酶活性研究。结果表明，水辣蓼富含有效的生物活性化合物，需要通过分离和结构解析进行进一步研究，以找到用于治疗各种神经系统疾病的新型和负担得起的化合物。
We investigated Polygonum hydropiper L. (P. hydropiper) for phenolic contents, antioxidant, anticholinesterase activities, in an attempt to rationalize its use in neurological disorders. Plant crude extract (Ph.Cr), its subsequent fractions: n-hexane (Ph.Hex), chloroform (Ph.Chf), ethyl acetate (Ph.EtAc), n-Butanol (Ph.Bt), aqueous (Ph.Aq) and saponins (Ph.Sp) were evaluated for 1,1-diphenyl,2-picrylhydrazyl (DPPH), 2,2-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS) free radical scavenging potential. Further, acetylcholinesterase (AChE) & butyrylcholinesterase (BChE) inhibitory activities were performed using Ellman's assay. Moreover, total phenolic contents of plant extracts were determined and expressed in mg of gallic acid equivalent per gram of dry sample (mg GAE/g dry weight). Among different fractions, Ph.Cr (90.82), Ph.Chf (178.16), Ph.EtAc (203.44) and Ph.Bt (153.61) exhibited high phenolic contents. All fractions showed concentration dependent DPPH scavenging activity, with Ph.EtAc 71.33% (IC50 15 μg/ml), Ph.Bt 71.40% (IC50 3 μg/ml) and Ph.Sp 71.40% (IC50 35 μg/ml) were most potent. The plant extracts exhibited high ABTS scavenging ability i.e. Ph.Bt (91.03%), Ph.EtAc (90.56%), Ph.Sp (90.84%), Ph.Aq (90.56%) with IC50 < 0.01 μg/ml. All fractions showed moderate to high AChE inhibitory activity as; Ph.Cr, 86.87% (IC50 330 μg/ml), Ph.Hex, 87.49% (IC50 35 μg/ml), Ph.Chf, 84.76% (IC50 55 μg/ml), Ph.Sp, 87.58% (IC50 108 μg/ml) and Ph.EtAc 79.95% (IC50 310 μg/ml) at 1 mg/ml). Furthermore the BChE inhibitory activity was most prominent in Ph.Hex 90.30% (IC50 40 μg/ml), Ph.Chf 85.94% (IC50 215 μg/ml), Ph.Aq 87.62% (IC50 3 μg/ml) and Ph.EtAc 81.01% (IC50 395 μg/ml) fractions. In this study, for the first time, we determined phenolic contents, isolated crude saponins, investigated antioxidant and anticholinestrase potential of P. hydropiper extracts. The results indicate that P. hydropiper is enriched with potent bioactive compounds and warrant further investigation by isolation and structural elucidation to find novel and affordable compounds for the treatment of various neurological disorders.
登录
查看更多内容
影响因子：
5.8
作者：
ELLMAN, GL;COURTNEY, KD;FEATHERSTONE, RM
通讯作者：
FEATHERSTONE, RM
影响因子：
120.7
作者：
KNAPP, MJ;KNOPMAN, DS;KELLEY, CK
通讯作者：
KELLEY, CK
影响因子：
3.6
作者：
Li, X;Matsumoto, K;Kadota, S
通讯作者：
Kadota, S
影响因子：
--
作者：
Hussain, Iqbal;Khattak, Moneeb Ur Rehman;Haider, Sajjad
通讯作者：
Haider, Sajjad
影响因子：
5.4
作者：
Ingkaninan, K;Temkitthawon, P;Thongnoi, W
通讯作者：
Thongnoi, W