Yvh1 is required for a late maturation step in the 60S biogenesis pathway.
Yvh1 is required for a late maturation step in the 60S biogenesis pathway.
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DOI：
10.1083/jcb.200904111
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发表时间：
2009-09-21
期刊：
影响因子：
--
通讯作者：
Panse VG
中科院分区：
文献类型：
--
作者：
Kemmler S;Occhipinti L;Veisu M;Panse VG
揭示了从细胞核中的前核糖体到细胞质中的具有分裂能力的60 S核糖体亚基的逐步组装过程（也参见Lo等在本期中）。在进入翻译之前，前核糖体颗粒经历连续的晚期成熟步骤。在前60 S颗粒的情况下，这些步骤涉及穿梭成熟因子和转运受体的释放。在这项研究中，我们报告了一个新的成熟步骤中的60 S生物合成途径在芽殖酵母。我们表明，核仁/核核糖体样蛋白Mrt 4（同源的酸性核糖体P-蛋白Rpp 0）从前60 S颗粒的有效释放需要高度保守的蛋白Yvh 1，它只与晚期前60 S颗粒。细胞生物学和生物化学分析表明，Mrt 4不能与yvh 1 Δ细胞中晚期前60 S颗粒分离，诱导了yvh 1 Δ细胞核前核糖体RNA加工的延迟和前60 S输出缺陷。此外，我们已经分离出Mrt 4的功能等位基因的获得，这些等位基因特异性地绕过了对Yvh 1的需求，并挽救了所有yvh 1 Δ相关的表型。总之，我们的数据表明，Yvh 1介导的释放Mrt 4前60 S颗粒的Rpp 0细胞质加载，是一个强制性的后期步骤建设的precaution-competent 60 S亚基。
The step by step assembly process from preribosome in the nucleus to translation-competent 60S ribosome subunit in the cytoplasm is revealed (also see Lo et al. in this issue). Before entering translation, preribosomal particles undergo sequential late maturation steps. In the case of pre-60S particles, these steps involve the release of shuttling maturation factors and transport receptors. In this study, we report a new maturation step in the 60S biogenesis pathway in budding yeast. We show that efficient release of the nucleolar/nuclear ribosomal-like protein Mrt4 (homologous to the acidic ribosomal P-protein Rpp0) from pre-60S particles requires the highly conserved protein Yvh1, which associates only with late pre-60S particles. Cell biological and biochemical analyses reveal that Mrt4 fails to dissociate from late pre-60S particles in yvh1Δ cells, inducing a delay in nuclear pre–ribosomal RNA processing and a pre-60S export defect in yvh1Δ cells. Moreover, we have isolated gain of function alleles of Mrt4 that specifically bypass the requirement for Yvh1 and rescue all yvh1Δ-associated phenotypes. Together, our data suggest that Yvh1-mediated release of Mrt4 precedes cytoplasmic loading of Rpp0 on pre-60S particles and is an obligatory late step toward construction of translation-competent 60S subunits.
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