Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus.
Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus.
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DOI：
10.3892/mmr.2017.8344
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发表时间：
2018-03
影响因子：
3.4
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Luo Q;Li X;Xu C;Zeng L;Ye J;Guo Y;Huang Z;Li J
成千上万的长链非编码RNA（lncRNA）已被报道，并代表了广泛的生物功能相关的普遍基因的重要子集。lncRNA的异常表达水平已在多种类型的人类疾病中得到证实。然而，lncRNA在系统性红斑狼疮（SLE）中的作用仍然知之甚少。在本研究中，使用Human lncRNA Array v3.0（8×60 K; Arraystar，Inc.，Rockville，MD，USA）。微阵列结果表明，与健康组相比，8，868个lncRNA（3，657个上调和5，211个下调）和6，876个mRNA（2，862个上调和4，014个下调）在SLE样品中高度差异表达。lncRNA靶标预测的基因本体（GO）分析表明，对于293个差异表达的lncRNA（倍数变化，≥3.0）和381个差异表达的mRNA（倍数变化，≥ 3.0），存在474个匹配的lncRNA-mRNA对。最丰富的途径是“癌症中的转录失调”和“缬氨酸、亮氨酸和异亮氨酸降解”。此外，逆转录-定量聚合酶链反应数据验证了6个异常的lncRNA和mRNA在SLE。结果表明，SLE患者lncRNA表达谱发生了显著变化。此外，还鉴定了一系列SLE相关的lncRNA。因此，本研究结果提供了重要的见解lncRNA在SLE的发病机制。
Thousands of long noncoding RNAs (lncRNAs) have been reported and represent an important subset of pervasive genes associated with a broad range of biological functions. Abnormal expression levels of lncRNAs have been demonstrated in multiple types of human disease. However, the role of lncRNAs in systemic lupus erythematosus (SLE) remains poorly understood. In the present study, the expression patterns of lncRNAs and messenger RNAs (mRNAs) were investigated in peripheral blood mononuclear cells (PBMCs) in SLE using Human lncRNA Array v3.0 (8×60 K; Arraystar, Inc., Rockville, MD, USA). The microarray results indicated that 8,868 lncRNAs (3,657 upregulated and 5,211 downregulated) and 6,876 mRNAs (2,862 upregulated and 4,014 downregulated) were highly differentially expressed in SLE samples compared with the healthy group. Gene ontology (GO) analysis of lncRNA target prediction indicated the presence of 474 matched lncRNA-mRNA pairs for 293 differentially expressed lncRNAs (fold change, ≥3.0) and 381 differentially expressed mRNAs (fold change, ≥3.0). The most enriched pathways were ‘Transcriptional misregulation in cancer’ and ‘Valine, leucine and isoleucine degradation’. Furthermore, reverse transcription-quantitative polymerase chain reaction data verified six abnormal lncRNAs and mRNAs in SLE. The results indicate that the lncRNA expression profile in SLE was significantly changed. In addition, a range of SLE-associated lncRNAs were identified. Thus, the present results provide important insights regarding lncRNAs in the pathogenesis of SLE.
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影响因子：
3.8
作者：
Mueller, Nike;Doering, Frank;Laudes, Matthias
通讯作者：
Laudes, Matthias
影响因子：
4.6
作者：
Song, Jinsoo;Kim, Dongkyun;Jin, Eun-Jung
通讯作者：
Jin, Eun-Jung
影响因子：
2.5
作者：
Liu, Dongmei;Zhao, Haiyan;Wang, Xiaofei
通讯作者：
Wang, Xiaofei
人类椎间盘退变中异常表达的长非编码 RNA：一项微阵列相关研究。
影响因子：
4.9
作者：
Wan ZY;Song F;Sun Z;Chen YF;Zhang WL;Samartzis D;Ma CJ;Che L;Liu X;Ali MA;Wang HQ;Luo ZJ
通讯作者：
Luo ZJ
影响因子：
16.8
作者：
Heward JA;Lindsay MA
通讯作者：
Lindsay MA