Association between MC1R gene and coat color segregation in Shanxia long black pig and Lulai black pig.
Association between MC1R gene and coat color segregation in Shanxia long black pig and Lulai black pig.
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DOI：
10.1186/s12863-023-01161-2
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发表时间：
2023-11-30
期刊：
影响因子：
1.9
通讯作者：
中科院分区：
文献类型：
--
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毛色作为猪的一个明显的表型特征，在新品种的选育过程中往往受到偏好和选择的影响。山西龙长黑猪是以伯克夏公猪和利查黑猪母猪为父本杂交选育而成，肉质优良，毛色黑色。杂交F_1代的毛色为黑色，但在以后的世代中分离。本研究旨在破解山西龙长黑猪毛色分离的遗传基础，建立一种区分黑猪和斑点猪的方法。仅在6号染色体的近端定位了一个QTL，并选择了MC1R基因作为功能候选基因。在MC1R基因上共检测到11个多态位点，只有C.67_68insCC变异与毛色共分离。该基因座不能被任何限制性内切酶识别，因此不能用PCR-RFLP进行基因分型。 >； A多态位点c.370G与毛色显著相关，且与c.67_68insCC紧密连锁不平衡。此外，它还得到了BspHI的认可。因此，建立了一种对该基因座进行基因分型的PCR-RFLP方法。除175头测序个体外，另有1,391头猪进行了PCR-RFLP基因分型，所有GG猪(一条带)都是黑色的。MC1R基因(c.67_68insCC)是导致毛色分离的基因(突变)，其扩增产物C.370G >； A可用于山西龙长黑猪的育种。网上版载有补充材料，可在10.1186/s12863-023-01161-2查阅。
Coat color, as a distinct phenotypic characteristic of pigs, is often subject to preference and selection, such as in the breeding process of new breed. Shanxia long black pig was derived from an intercross between Berkshire boars and Licha black pig sows, and it was bred as a paternal strain with high-quality meat and black coat color. Although the coat color was black in the F1 generation of the intercross, it segregated in the subsequent generations. This study aims to decode the genetic basis of coat color segregation and develop a method to distinct black pigs from the spotted in Shanxia long black pig. Only a QTL was mapped at the proximal end of chromosome 6, and MC1R gene was picked out as functional candidate gene. A total of 11 polymorphic loci were identified in MC1R gene, and only the c.67_68insCC variant was co-segregating with coat color. This locus isn’t recognized by any restriction endonuclease, so it can’t be genotyped by PCR-RFLP. The c.370G > A polymorphic locus was also significantly associated with coat color, and has been in tightly linkage disequilibrium with the c.67_68insCC. Furthermore, it is recognized by BspHI. Therefore, a PCR-RFLP method was set up to genotype this locus. Besides the 175 sequenced individuals, another more 1,391 pigs were genotyped with PCR-RFLP, and all of pigs with GG (one band) were black. MC1R gene (c.67_68insCC) is the causative gene (mutation) for the coat color segregation, and the PCR-RFLP of c.370G > A could be used in the breeding program of Shanxia long black pig. The online version contains supplementary material available at 10.1186/s12863-023-01161-2.
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影响因子：
3.7
作者：
Huang L;Dong BC;Xue W;Peng YK;Zhang MX;Yu FH
通讯作者：
Yu FH
DOI：
10.1186/1297-9686-14-4-481
发表时间：
1982
期刊：
Annales de genetique et de selection animale
影响因子：
--
作者：
Ollivier L;Sellier P
通讯作者：
Sellier P
MC1R 和 ASIP 之间的协同作用对马（Equus caballus）毛色的影响
影响因子：
3.3
作者：
Shang, Songyang;Yu, Yan;Wang, Zhe
通讯作者：
Wang, Zhe
影响因子：
3.8
作者：
Li, J.;Yang, H.;Zhang, Y-P
通讯作者：
Zhang, Y-P
影响因子：
5.1
作者：
Li Ya-lan;Zhang Qin;Chen Yao-sheng
通讯作者：
Chen Yao-sheng