Generation of Doubled Haploid Wheat-Triticum urartu Introgression Lines and Their Characterisation Using Chromosome-Specific KASP Markers.
Generation of Doubled Haploid Wheat-Triticum urartu Introgression Lines and Their Characterisation Using Chromosome-Specific KASP Markers.
复制标题
DOI：
10.3389/fpls.2021.643636
复制
发表时间：
2021
影响因子：
5.6
通讯作者：
King J
中科院分区：
文献类型：
--
作者：
Grewal S;Guwela V;Newell C;Yang CY;Ashling S;Scholefield D;Hubbart-Edwards S;Burridge A;Stride A;King IP;King J
小麦是世界上最重要的食物和蛋白质来源之一，尽管近年来小麦育种者实现了增产，但还不足以满足不断增长的人口的需求。由于小麦遗传基础狭窄，对气候变化造成的非生物和生物胁迫具有弹性的高产小麦品种的开发一直受到限制。与小麦相反，小麦的野生近亲为大多数(如果不是全部)农艺性状提供了巨大的遗传变异储藏库。作者之前的研究表明，T.urartu的遗传变异已转移到面包小麦中。然而，在利用导入系进行性状分析之前，需要将导入系稳定地传递给下一代。在本工作中，我们描述了86个加倍单倍体小麦-T。乌拉图导入系，携带稳定遗传的纯合导入系。使用Axiom®小麦相对基因分型阵列和151个KASP标记对这些DH品系进行了鉴定，以鉴定65个独特的小麦小麦背景中的T.urartu导入。水解氢的产生加快了育种进程，促进了纯合小麦-T的早期释放。乌拉图渗入线。与KASP标记一起，这一有价值的资源可以极大地促进可用于小麦改良的有益等位基因的鉴定。
Wheat is one of the most important food and protein sources in the world and although, in recent years wheat breeders have achieved yield gains, they are not sufficient to meet the demands of an ever-growing population. Development of high yielding wheat varieties, resilient to abiotic and biotic stress resulting from climate change, has been limited by wheat’s narrow genetic base. In contrast to wheat, the wild relatives of wheat provide a vast reservoir of genetic variation for most, if not all, agronomic traits. Previous studies by the authors have shown the transfer of genetic variation from T. urartu into bread wheat. However, before the introgression lines can be exploited for trait analysis, they are required to have stable transmission of the introgressions to the next generation. In this work, we describe the generation of 86 doubled haploid (DH) wheat-T. urartu introgression lines that carry homozygous introgressions which are stably inherited. The DH lines were characterised using the Axiom® Wheat Relative Genotyping Array and 151 KASP markers to identify 65 unique T. urartu introgressions in a bread wheat background. DH production has helped accelerate the breeding process and facilitated the early release of homozygous wheat-T. urartu introgression lines. Together with the KASP markers, this valuable resource could greatly advance identification of beneficial alleles that can be used in wheat improvement.
登录
查看更多内容
影响因子：
1.5
作者：
CHAPMAN, V;MILLER, TE;RILEY, R
通讯作者：
RILEY, R
通过全基因组渗入小麦的脑膜膜染色体表征。
DOI：
10.1007/s00122-017-3009-y
发表时间：
2018-03
期刊：
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
影响因子：
--
作者：
Grewal S;Yang C;Edwards SH;Scholefield D;Ashling S;Burridge AJ;King IP;King J
通讯作者：
King J
影响因子：
64.8
作者：
Ling HQ;Ma B;Shi X;Liu H;Dong L;Sun H;Cao Y;Gao Q;Zheng S;Li Y;Yu Y;Du H;Qi M;Li Y;Lu H;Yu H;Cui Y;Wang N;Chen C;Wu H;Zhao Y;Zhang J;Li Y;Zhou W;Zhang B;Hu W;van Eijk MJT;Tang J;Witsenboer HMA;Zhao S;Li Z;Zhang A;Wang D;Liang C
通讯作者：
Liang C
影响因子：
5.6
作者：
Grewal S;Hubbart-Edwards S;Yang C;Scholefield D;Ashling S;Burridge A;Wilkinson PA;King IP;King J
通讯作者：
King J
DOI：
10.1073/pnas.072223799
发表时间：
2002-06-11
影响因子：
11.1
作者：
Huang, S;Sirikhachornkit, A;Gornicki, P
通讯作者：
Gornicki, P