Identification of resistant germplasm containing novel resistance genes at or tightly linked to the Pi2/9 locus conferring broad-spectrum resistance against rice blast.
Identification of resistant germplasm containing novel resistance genes at or tightly linked to the Pi2/9 locus conferring broad-spectrum resistance against rice blast.
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DOI：
10.1186/s12284-017-0176-z
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发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
Zhou B
中科院分区：
文献类型：
--
作者：
Xiao G;Borja FN;Mauleon R;Padilla J;Telebanco-Yanoria MJ;Yang J;Lu G;Dionisio-Sese M;Zhou B
水稻Pi2/9基因座含有多个抗性(R)基因，每个基因都控制着对水稻稻瘟病菌不同分离物的广谱抗性。在Pi2/9基因座或与Pi2/9基因座紧密连锁的抗病种质中发现更多含有新R基因的抗病种质，将有助于抗病品种的选育。在本研究中，我们旨在利用一个分子标记Pi2/9-RH(Pi2/9抗性单倍型)来鉴定含有新的R基因的抗病种质，该标记仅在含有功能Pi2/9等位基因的水稻品系中保守。用PI2/9-RH进行DNA分析，在入围的55个地方品种中鉴定出24个阳性品系，表现出对4个稻瘟病分离物的抗性。对PI2/9同源基因全长cDNA的部分序列进行分析，将这24个品系聚为5个单倍型，每个单倍型包含不同的PI2/9同源基因，分别命名为PI2/9-A5、−A15、−A42、−A53和-A54。有趣的是，Pi2/9-A5和Pi2/9-A54分别与Piz-t和Pi2相同。为了验证其他3个新的Pi2/9同源基因与稻瘟病抗性的关联，通过标记辅助回交(MABC)获得了BC3F3世代的单基因系。在温室和田间热点地区对衍生的单基因系进行抗性鉴定表明，它们都控制了对稻瘟病的广谱抗性。此外，遗传分析表明，这3个单基因系的稻瘟病抗性是与Pi2/9-RH共分离的，这表明Pi2/9基因座或紧密连锁的基因座可能与稻瘟病抗性有关。新开发的标记Pi2/9-RH可作为一种潜在的诊断标记，用于快速鉴定含有功能性Pi2/9等位基因或未知连锁R基因的抗性供体。含有Pi2/9导入片段的3个新的单基因系可作为抗病鉴定和育种工作中有价值的材料。本文的在线版本(doi：10.1186/s12284-0176z)包含补充材料，授权用户可以使用。
The rice Pi2/9 locus harbors multiple resistance (R) genes each controlling broad-spectrum resistance against diverse isolates of Magnaporthe oryzae, a fungal pathogen causing devastating blast disease to rice. Identification of more resistance germplasm containing novel R genes at or tightly linked to the Pi2/9 locus would promote breeding of resistance rice cultivars. In this study, we aim to identify resistant germplasm containing novel R genes at or tightly linked to the Pi2/9 locus using a molecular marker, designated as Pi2/9-RH (Pi2/9 resistant haplotype), developed from the 5′ portion of the Pi2 sequence which was conserved only in the rice lines containing functional Pi2/9 alleles. DNA analysis using Pi2/9-RH identified 24 positive lines in 55 shortlisted landraces which showed resistance to 4 rice blast isolates. Analysis of partial sequences of the full-length cDNAs of Pi2/9 homologues resulted in the clustering of these 24 lines into 5 haplotypes each containing different Pi2/9 homologues which were designated as Pi2/9-A5, −A15, −A42, −A53, and -A54. Interestingly, Pi2/9-A5 and Pi2/9-A54 are identical to Piz-t and Pi2, respectively. To validate the association of other three novel Pi2/9 homologues with the blast resistance, monogenic lines at BC3F3 generation were generated by marker assisted backcrossing (MABC). Resistance assessment of the derived monogenic lines in both the greenhouse and the field hotspot indicated that they all controlled broad-spectrum resistance against rice blast. Moreover, genetic analysis revealed that the blast resistance of these three monogenic lines was co-segregated with Pi2/9-RH, suggesting that the Pi2/9 locus or tightly linked loci could be responsible for the resistance. The newly developed marker Pi2/9-RH could be used as a potentially diagnostic marker for the quick identification of resistant donors containing functional Pi2/9 alleles or unknown linked R genes. The three new monogenic lines containing the Pi2/9 introgression segment could be used as valuable materials for disease assessment and resistance donors in breeding program. The online version of this article (doi:10.1186/s12284-017-0176-z) contains supplementary material, which is available to authorized users.
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影响因子：
5.4
作者：
Hayashi, K;Hashimoto, N;Ashikawa, I
通讯作者：
Ashikawa, I
影响因子：
5.2
作者：
Costanzo, Stefano;Jia, Yulin
通讯作者：
Jia, Yulin
影响因子：
3.2
作者：
Campbell, MA;Chen, D;Ronald, PC
通讯作者：
Ronald, PC
影响因子：
5.4
作者：
Hua, Lixia;Wu, Jianzhong;Pan, Qinghua
通讯作者：
Pan, Qinghua
Pi2/9 等位基因 Pi2-2 的分子图谱赋予水稻品种 Jefferson 对稻瘟病的广谱抗性
DOI：
10.1186/1939-8433-5-29
发表时间：
2012-12
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Jiang N;Li Z;Wu J;Wang Y;Wu L;Wang S;Wang D;Wen T;Liang Y;Sun P;Liu J;Dai L;Wang Z;Wang C;Luo M;Liu X;Wang GL
通讯作者：
Wang GL