Silk hydrogel for cartilage tissue engineering.
Silk hydrogel for cartilage tissue engineering.
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DOI：
10.1002/jbm.b.31686
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发表时间：
2010-10
影响因子：
3.4
通讯作者：
Vunjak-Novakovic, Gordana
中科院分区：
文献类型：
--
作者：
Chao, Pen-Hsiu Grace;Yodmuang, Supansa;Wang, Xiaoqin;Sun, Lin;Kaplan, David L.;Vunjak-Novakovic, Gordana
基于在琼脂糖水凝胶中培养未成熟软骨细胞的软骨组织工程能够产生具有与天然软骨相当的生物力学性能的组织构建体。然而，琼脂糖具有免疫原性且不可降解，我们改变这种材料的结构、成分和力学性能的能力相当有限。相比之下，丝水凝胶具有生物相容性且可生物降解，并且可以使用无有机溶剂的水基方法生产，这使得能够在软骨组织工程所关注的范围内精确控制结构和力学性能。我们观察到一种特定的丝水凝胶制剂所产生的软骨构建体的生化成分和力学性能与基于琼脂糖的构建体相匹配。这一发现以及改变丝水凝胶性能的可能性促使了这项关于水凝胶对软骨组织工程适用性的潜在因素的研究。我们展示了丝水凝胶性能、丝提取方法、凝胶浓度和凝胶结构的系统性变化所产生的数据。数据表明，丝水凝胶可作为研究与水凝胶相关的参与软骨形成的因素和机制的工具，以及作为软骨组织工程中一种可定制且完全可降解的支架。
Cartilage tissue engineering based on cultivation of immature chondrocytes in agarose hydrogel can yield tissue constructs with biomechanical properties comparable to native cartilage. However, agarose is immunogenic and non-degradable, and our capability to modify the structure, composition, and mechanical properties of this material is rather limited. In contrast, silk hydrogel is biocompatible and biodegradable, and it can be produced using a water-based method without organic solvents that enables precise control of structural and mechanical properties in a range of interest for cartilage tissue engineering. We observed that one particular preparation of silk hydrogel yielded cartilaginous constructs with biochemical content and mechanical properties matching constructs based on agarose. This finding and the possibility to vary the properties of silk hydrogel motivated this study of the factors underlying the suitability of hydrogels for cartilage tissue engineering. We present data resulting from a systematic variation of silk hydrogel properties, silk extraction method, gel concentration, and gel structure. Data suggest that silk hydrogel can be used as a tool for studies of the hydrogel-related factors and mechanisms involved in cartilage formation, as well as a tailorable and fully degradable scaffold for cartilage tissue engineering.
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DOI：
10.1115/1.1318906
发表时间：
2000-12-01
影响因子：
1.7
作者：
Butler, DL;Goldstein, SA;Guilak, F
通讯作者：
Guilak, F
影响因子：
2.4
作者：
Albro, Michael B.;Chahine, Nadeen O.;Li, Roland;Yeager, Keith;Hung, Clark T.;Ateshian, Gerard A.
通讯作者：
Ateshian, Gerard A.
影响因子：
--
作者：
Hofmann, Sandra;Knecht, Sven;Meinel, Lorenz
通讯作者：
Meinel, Lorenz
DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ
DOI：
10.1115/1.429656
发表时间：
2000-06-01
影响因子：
1.7
作者：
Mauck, RL;Soltz, MA;Ateshian, GA
通讯作者：
Ateshian, GA