A comparison of hemagglutination inhibition and neutralization assays for characterizing immunity to seasonal influenza A.
A comparison of hemagglutination inhibition and neutralization assays for characterizing immunity to seasonal influenza A.
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DOI：
10.1111/irv.12408
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发表时间：
2016-11
影响因子：
4.4
通讯作者：
Cummings DA
中科院分区：
文献类型：
--
作者：
Truelove S;Zhu H;Lessler J;Riley S;Read JM;Wang S;Kwok KO;Guan Y;Jiang CQ;Cummings DA
流感血清抗体可用于识别过去的暴露和测量当前的免疫状态。两种最常用的测量方法是血凝抑制试验（HI）和病毒中和试验（NT），尚未对大量流感病毒进行系统比较。2009年，共有151名来自中国广州附近的研究参与者入组并提供血清。对12种历史和近期流行的季节性甲型流感病毒株进行HI和NT检测。我们使用斯皮尔曼相关性比较滴度，并使用HI结果拟合模型预测NT。我们在所有菌株中观察到HI和NT测定之间的高度正平均相关性（斯皮尔曼秩相关，ρ= 0.86）。在亚型内和在时间上接近的范围内相关性最高。总体而言，HI=20对应于NT=10，HI=40对应于NT=20。log（NT）对log（HI）的线性回归具有统计学显著性，年龄改变了这种关系。菌株特异性曲线下面积（AUC）表明预测HI NT的准确性良好（>80%）。虽然我们发现季节性甲型流感病毒的NT和HI检测之间的滴度总体一致性较高，但无法确定检测之间的确切等效性。滴度随年龄变化之间的对应关系使这变得更加复杂。这些发现支持对检测试剂之间的结果进行一般性比较，并进一步支持使用血凝抑制检测试剂而非资源密集型病毒中和检测试剂检测季节性甲型流感，但应注意年龄对这些检测试剂的影响。
Serum antibody to influenza can be used to identify past exposure and measure current immune status. The two most common methods for measuring this are the hemagglutination inhibition assay (HI) and the viral neutralization assay (NT), which have not been systematically compared for a large number of influenza viruses. A total of 151 study participants from near Guangzhou, China, were enrolled in 2009 and provided serum. HI and NT assays were performed for 12 historic and recently circulating strains of seasonal influenza A. We compared titers using Spearman correlation and fit models to predict NT using HI results. We observed high positive mean correlation between HI and NT assays (Spearman's rank correlation, ρ=.86) across all strains. Correlation was highest within subtypes and within close proximity in time. Overall, an HI=20 corresponded to NT=10, and HI=40 corresponded to NT=20. Linear regression of log(NT) on log(HI) was statistically significant, with age modifying this relationship. Strain‐specific area under a curve (AUC) indicated good accuracy (>80%) for predicting NT with HI. While we found high overall correspondence of titers between NT and HI assays for seasonal influenza A, no exact equivalence between assays could be determined. This was further complicated by correspondence between titers changing with age. These findings support generalized comparison of results between assays and give further support for use of the hemagglutination inhibition assay over the more resource intensive viral neutralization assay for seasonal influenza A, although attention should be given to the effect of age on these assays.
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