Piscirickettsia salmonis-Triggered Extracellular Traps Formation as an Innate Immune Response of Atlantic Salmon-Derived Polymorphonuclear Neutrophils.
Piscirickettsia salmonis-Triggered Extracellular Traps Formation as an Innate Immune Response of Atlantic Salmon-Derived Polymorphonuclear Neutrophils.
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DOI：
10.3390/biology10030206
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发表时间：
2021-03-09
期刊：
影响因子：
4.2
通讯作者：
Morera FJ
中科院分区：
文献类型：
--
作者：
Alarcon P;Espinosa G;Millan C;Saravia J;Quinteros V;Enriquez R;Henriquez C;Vargas-Chacoff L;Burgos RA;Taubert A;Hermosilla C;Morera FJ
在先天免疫中，多形核中性粒细胞（PMN）是最丰富的白细胞群体。除了PMN之外，还已知存在单核细胞、嗜酸性粒细胞和嗜碱性粒细胞。所有这些都可以释放细胞外陷阱（Extracellular trap，缩写为ETP），这是一种复杂的网状结构，由核组蛋白、颗粒酶、肽和蛋白质修饰的染色质组成，可以牢固地捕获入侵病原体，从而减缓传播并有助于产生针对细菌、真菌、病毒和寄生虫的适当免疫反应。在这里，我们第一次表明，大西洋鲑鱼来源的PMN释放ET样结构在体外，在高致病性兼性细胞内立克次体细菌鲑鱼Piscirickettsia。从PMN中释放ET样结构可能是提高养殖鲑鱼对病原体防御能力的新选择。细胞外陷阱是由DNA、瓜氨酸化组蛋白、抗微生物肽和蛋白质组成的网，这些在以前的大西洋鲑鱼（萨尔莫salar）中没有报道。主要由多形核中性粒细胞（PMN）释放，被认为是一种新的PMN衍生的效应机制，针对不同的侵入性病原体。在这里，我们表明，大西洋鲑鱼来源的PMN释放ET样结构在体外高致病性兼性细胞内立克次体细菌Piscirickettsia鲑。中性粒细胞分离前鲑鱼和刺激在体外与油酸和P.鲑鱼。使用PicoGreen™染料测量细胞外DNA，同时使用免疫荧光图像分析来确认鲑鱼挤出的鲑鱼的经典组分。未来的研究需要更好地了解大西洋鲑鱼源性免疫系统协调先天/适应性免疫的作用，以及参与这种细胞死亡过程的调控途径的知识。因此，鲑鱼源性立克次体对P. salmonis的理解可能代表了新的替代策略，以改善养殖鲑鱼对密切相关的立克次体细菌的宿主先天防御机制，作为疾病预防和控制策略的补充。
Within innate immunity, polymorphonuclear neutrophils (PMN) are the most abundant leukocyte population. Alongside PMN, monocytes, eosinophils, and basophils are also known to exist. All of them can release extracellular traps (ETs), a complex web-like structure composed of chromatin decorated with nuclear histones, granular enzymes, peptides, and proteins, to firmly entrap invasive pathogens, thereby slowing dissemination and helping to develop proper immune responses against bacteria, fungi, viruses, and parasites. Here, we showed for the first time that Atlantic salmon-derived PMN released ETs-like structures in vitro, in response to highly pathogenic facultative intracellular rickettsial bacteria Piscirickettsia salmonis. The release of ET-like structures from PMN could be a new alternative to improve farmed salmon’s defense against pathogens. Extracellular traps (ETs) are webs of DNA, citrullinated histones, anti-microbial peptides, and proteins that were not previously reported in Atlantic salmon (Salmo salar). ETs are mainly released from polymorphonuclear neutrophils (PMN) and are considered a novel PMN-derived effector mechanism against different invasive pathogens. Here, we showed that Atlantic salmon-derived PMN released ETs-like structures in vitro in response to highly pathogenic facultative intracellular rickettsial bacteria Piscirickettsia salmonis. PMN were isolated from pre-smolt Atlantic salmon and stimulated in vitro with oleic acid and P. salmonis. Extracellular DNA was measured using the PicoGreen™ dye, while immunofluorescence image analysis was used to confirm the classical components of salmonid-extruded ETs. Future studies are required to better understand the role of Atlantic salmon-derived ETs orchestrating innate/adaptive immunity and the knowledge on regulation pathways involved in this cell death process. Thus, comprehension of salmonid-derived ETs against P. salmonis might represent novel alternative strategies to improve host innate defense mechanisms of farmed salmon against closely related rickettsial bacteria, as a complement to disease prevention and control strategies.
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影响因子：
2.5
作者：
BRANSON, EJ;DIAZMUNOZ, DN
通讯作者：
DIAZMUNOZ, DN
影响因子：
1
作者：
Burgos, RA;Hidalgo, MA;Hancke, JL
通讯作者：
Hancke, JL
影响因子：
2.4
作者：
Conejeros, I.;Jara, E.;Burgos, R. A.
通讯作者：
Burgos, R. A.
DOI：
10.1590/0001-3765201820170730
发表时间：
2018-12-01
期刊：
Anais da Academia Brasileira de Ciências
影响因子：
--
作者：
BILLER, JAQUELINE D.;TAKAHASHI, LEONARDO S.
通讯作者：
TAKAHASHI, LEONARDO S.
影响因子：
5.5
作者：
Havixbeck, Jeffrey J.;Rieger, Aja M.;Barreda, Daniel R.
通讯作者：
Barreda, Daniel R.