Campylobacter jejuni Developed the Resistance to Bacteriophage CP39 by Phase Variable Expression of 06875 Encoding the CGPTase.
Campylobacter jejuni Developed the Resistance to Bacteriophage CP39 by Phase Variable Expression of 06875 Encoding the CGPTase.
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空肠弯曲杆菌通过编码 CGPTase 的 06875 的相变表达产生对噬菌体 CP39 的抗性
DOI：
10.3390/v14030485
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发表时间：
2022-02-26
期刊：
影响因子：
--
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Tang Y;Li J;Wang Y;Song Z;Ying H;Kong L;Jiao X;Huang J
噬菌体（噬菌体）被认为是食品生产中弯曲杆菌的抗菌替代品。然而，噬菌体对宿主的抗性的发展是噬菌体应用的主要关注点。本研究对噬菌体 CP39 进行了表征，并研究了空肠弯曲杆菌 NCTC12662 中 CP39 的噬菌体抗性。通过全基因组测序和系统发育分析，我们确定噬菌体 CP39 属于肌病毒科。通过初级空肠弯曲菌噬菌体分型，证实噬菌体 CP39 是荚膜多糖 (CPS) 依赖性噬菌体。进一步证实，噬菌体不能被无荚膜突变体ΔkpsM吸附，但在野生型菌株NCTC 12662和缺乏运动鞭毛丝的ΔmotA突变体中表现出相同的裂解能力。通过SNP分析，我们进一步确定CPS位点中编码CDP-甘油：聚（甘油磷酸）甘油磷酸转移酶（CGPTase）的06875基因与噬菌体CP39吸附有关，并观察到噬菌体感染期间NCTC 12662中噬菌体抗性的快速发展。此外，我们观察到06875（32%）的高突变频率，根据菌落PCR测序，该突变随机发生在基因的9个不同位点。 06875基因的突变可引起非功能蛋白的相变表达，使细菌通过修饰CPS来抵抗噬菌体感染。我们的研究首次证实了负责CPS-噬菌体吸附的06875基因，并证明了相变表达是细菌防御噬菌体CP39的主要机制。我们的研究为细菌针对噬菌体的进化适应提供了知识，这可以在应用于工业之前添加更多信息来了解噬菌体抵抗机制。
Bacteriophage (phage) is regarded as an antimicrobial alternative for Campylobacter in food production. However, the development of phage resistance to the host is a main concern for the phage application. This study characterized the phage CP39 and investigated the phage resistance of CP39 in Campylobacter jejuni NCTC12662. We determined that phage CP39 belonged to the Myoviridae family by the WGS and phylogenetic analysis. Phage CP39 was confirmed as a capsular polysaccharide (CPS)-dependent phage by primary C. jejuni phage typing. It was further confirmed that the phage could not be adsorbed by the acapsular mutant ΔkpsM but showed the same lytic ability in both the wild-type strain NCTC 12662 and the ΔmotA mutant lacking motile flagella filaments. We further determined that the 06875 gene encoding CDP-glycerol:poly (glycerophosphate) glycerophosphotransferase (CGPTase) in the CPS loci was related to phage CP39 adsorption by SNP analysis and observed a rapid development of phage resistance in NCTC 12662 during the phage infection. Furthermore, we observed a high mutation frequency of 06875 (32%), which randomly occurred in nine different sites in the gene according to colony PCR sequencing. The mutation of the 06875 gene could cause the phase variable expression of non-functional protein and allow the bacteria against the phage infection by modifying the CPS. Our study confirmed the 06875 gene responsible for the CPS-phage adsorption for the first time and demonstrated the phase variable expression as a main mechanism for the bacteria to defend phage CP39. Our study provided knowledge for the evolutionary adaption of bacteria against the bacteriophage, which could add more information to understand the phage resistance mechanism before applying in the industry.
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影响因子：
5.7
作者：
Holst Sørensen MC;van Alphen LB;Fodor C;Crowley SM;Christensen BB;Szymanski CM;Brøndsted L
通讯作者：
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4.3
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作者：
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DOI：
10.3390/ijerph10126292
发表时间：
2013-11-26
影响因子：
--
作者：
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通讯作者：
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影响因子：
6.7
作者：
Li, Xiaofei;Chai, Qinwen;Huang, Jinlin
通讯作者：
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