Biogeographic patterns of biosynthetic potential and specialized metabolites in marine sediments.
Biogeographic patterns of biosynthetic potential and specialized metabolites in marine sediments.
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DOI：
10.1038/s41396-023-01410-3
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发表时间：
2023-07
期刊：
影响因子：
11
通讯作者：
Jensen, Paul R.
中科院分区：
文献类型：
--
作者：
Chase, Alexander B.;Bogdanov, Alexander;Demko, Alyssa M.;Jensen, Paul R.
虽然微生物群落学领域主要集中在非生物因素对群落模式的贡献上，但生物相互作用在构建微生物群落中的潜在影响，例如由专门代谢产物的产生介导的那些，在很大程度上仍然未知。在这里，我们研究了微生物群落结构和专门的代谢之间的关系，在当地的空间尺度上的海洋沉积物样品收集的长期生态研究（LTER）网站在莫雷阿，法属波利尼西亚。通过采用多组学方法来表征沉积物群落中的分类、功能和专门的代谢物组成，我们发现沉积物的地理模式是由局部尺度过程驱动的（例如，生物相互作用），并在很大程度上独立于扩散限制。具体而言，我们观察到样品之间的生物合成潜力（基于Bray-Curtis相异性）的高度变化，即使在1平方米的地块内，也反映了与位点特异性代谢组相关的未表征化学空间。最终，连接生物合成潜力社区代谢促进了天然产物的原位检测，并揭示了新的见解与沉积物微生物群落的复杂代谢动力学。我们的研究证明了将生物合成基因和代谢产物生产整合到微生物群落动态评估中的潜力。
While the field of microbial biogeography has largely focused on the contributions of abiotic factors to community patterns, the potential influence of biotic interactions in structuring microbial communities, such as those mediated by the production of specialized metabolites, remains largely unknown. Here, we examined the relationship between microbial community structure and specialized metabolism at local spatial scales in marine sediment samples collected from the Long-Term Ecological Research (LTER) site in Moorea, French Polynesia. By employing a multi-omic approach to characterize the taxonomic, functional, and specialized metabolite composition within sediment communities, we find that biogeographic patterns were driven by local scale processes (e.g., biotic interactions) and largely independent of dispersal limitation. Specifically, we observed high variation in biosynthetic potential (based on Bray-Curtis dissimilarity) between samples, even within 1 m2 plots, that reflected uncharacterized chemical space associated with site-specific metabolomes. Ultimately, connecting biosynthetic potential to community metabolomes facilitated the in situ detection of natural products and revealed new insights into the complex metabolic dynamics associated with sediment microbial communities. Our study demonstrates the potential to integrate biosynthetic genes and metabolite production into assessments of microbial community dynamics.
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DOI：
10.1073/pnas.2212930119
发表时间：
2022-10-18
影响因子：
11.1
作者：
通讯作者：
--
影响因子：
6.4
作者：
Choudoir, Mallory;Rossabi, Sam;Fierer, Noah
通讯作者：
Fierer, Noah
影响因子：
3.3
作者：
Liu, Jie;Lopez, Nora;Haeggblom, Max M.
通讯作者：
Haeggblom, Max M.
影响因子：
56.9
作者：
Lima-Mendez, Gipsi;Faust, Karoline;Wincker, Patrick
通讯作者：
Wincker, Patrick
DOI：
10.1073/pnas.1318021111
发表时间：
2014-03-11
影响因子：
11.1
作者：
Charlop-Powers, Zachary;Owen, Jeremy G.;Brady, Sean F.
通讯作者：
Brady, Sean F.