MYB57 transcriptionally regulates MAPK11 to interact with PAL2;3 and modulate rice allelopathy
MYB57 transcriptionally regulates MAPK11 to interact with PAL2;3 and modulate rice allelopathy
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MYB57 转录调节 MAPK11 与 PAL2;3 相互作用并调节水稻化感作用
DOI：
10.1093/jxb/erz540
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发表时间：
2019-12
影响因子：
6.9
通讯作者：
Wenxiong Lin
中科院分区：
文献类型：
--
作者：
Changxun Fang;Luke Yang;Weisi Chen;Lanlan Li;Pengli Zhang;Yingzhe Li;Haibin He;Wenxiong Lin
摘要水稻化感作用是一种自然的除草方法，在农业生态学中被认为是一种生态友好的措施。水稻的化感作用受多种基因调控，包括转录因子。本研究对MYB转录因子OsMYB 57进行了鉴定，以探讨其在水稻化感作用调控中的作用。使用转录激活剂VP 64增加OsMYB 57在水稻中的表达导致对稗草的抑制率增加。苯丙烷代谢途径中的OsPAL、OsC 4 H、OsOMT和OsCAD基因表达水平上调，l-苯丙氨酸含量增加。染色质免疫沉淀结合HiSeq技术证实OsMYB 57转录调控丝裂原活化蛋白激酶（OsMAPK 11），OsMAPK 11与OsPAL 2相互作用。OsPAL 2的表达;化感水稻PI 312777中OsPAL 2; 3的表达量高于非化感水稻Lemont，OsPAL 2;3受Whirly转录因子负调控。此外，具有杂草抑制潜力的微生物，包括青霉属（Penicillium spp.）和芽孢杆菌属，OsMYB 57基因在水稻Kitaake的根际表达增加，参与了酚酸的诱导。我们的研究结果表明，OsMYB 57正调控水稻化感作用，为通过遗传修饰改良水稻化感性状提供了一种选择。
Abstract Rice allelopathy is a natural method of weed control that is regarded as an eco-friendly practice in agroecology. The allelopathic potential of rice is regulated by various genes, including those that encode transcription factors. Our study characterized a MYB transcription factor, OsMYB57, to explore its role in the regulation of rice allelopathy. Increasing the expression of OsMYB57 in rice using the transcription activator VP64 resulted in increased inhibitory ratios against barnyardgrass. The gene expression levels of OsPAL, OsC4H, OsOMT, and OsCAD from the phenylpropanoid pathway were also up-regulated, and the content of l-phenylalanine increased. Chromatin immunoprecipitation incorporated with HiSeq demonstrated that OsMYB57 transcriptionally regulated a mitogen-activated protein kinase (OsMAPK11); in addition, OsMAPK11 interacted with OsPAL2;3. The expression of OsPAL2;3was higher in the allelopathic rice PI312777 than in the non-allelopathic rice Lemont, and OsPAL2;3 was negatively regulated by Whirly transcription factors. Moreover, microbes with weed-suppression potential, including Penicillium spp. and Bacillus spp., were assembled in the rhizosphere of the rice accession Kitaake with increased expression of OsMYB57, and were responsible for phenolic acid induction. Our findings suggest that OsMYB57 positively regulates rice allelopathy, providing an option for the improvement of rice allelopathic traits through genetic modification.
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