Myo-Inositol Safety in Pregnancy: From Preimplantation Development to Newborn Animals.
Myo-Inositol Safety in Pregnancy: From Preimplantation Development to Newborn Animals.
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DOI：
10.1155/2016/2413857
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发表时间：
2016
影响因子：
2.8
通讯作者：
Bevilacqua A
中科院分区：
文献类型：
--
作者：
Kuşcu N;Bizzarri M;Bevilacqua A
肌醇（myo-Ins）在哺乳动物配子发生和胚胎发育中具有生理作用，并且对人类医学辅助生殖具有积极的临床影响。我们以前已经表明，小鼠胚胎暴露于myo-Ins通过植入前发育在体外增加增殖活性和囊胚生产，代表培养条件的改善。我们在此研究了肌胰岛素引起的植入前胚胎的生化机制，并评估肌胰岛素对植入后/出生后发育的影响。为此，在存在或不存在myo-Ins的情况下，将自然受精的胚胎体外培养至囊胚，并分析PKB/Akt途径的激活，已知PKB/Akt途径调节增殖/存活细胞过程。同时，将囊胚期胚胎移植到假孕雌性动物中，并使其发育至足月和断奶。获得的结果提供了证据表明myo-Ins诱导涉及Akt的细胞途径，并表明（a）植入前胚胎暴露于myo-Ins增加了可用于子宫移植的囊胚数量和交付动物的数量，（B）从存在或不存在myo-Ins的情况下培养的胚胎中获得的小鼠的发育模式，最多三周龄，重叠。这些数据进一步确定myo-Ins作为辅助生殖技术中人类植入前胚胎培养的可能重要补充。
Myo-inositol (myo-Ins) has a physiological role in mammalian gametogenesis and embryonic development and a positive clinical impact on human medically assisted reproduction. We have previously shown that mouse embryo exposure to myo-Ins through preimplantation development in vitro increases proliferation activity and blastocyst production, representing an improvement in culture conditions. We have herein investigated biochemical mechanisms elicited by myo-Ins in preimplantation embryos and evaluated myo-Ins effects on postimplantation/postnatal development. To this end naturally fertilized embryos were cultured in vitro to blastocyst in the presence or absence of myo-Ins and analyzed for activation of the PKB/Akt pathway, known to modulate proliferation/survival cellular processes. In parallel, blastocyst-stage embryos were transferred into pseudopregnant females and allowed to develop to term and until weaning. Results obtained provide evidence that myo-Ins induces cellular pathways involving Akt and show that (a) exposure of preimplantation embryos to myo-Ins increases the number of blastocysts available for uterine transfer and of delivered animals and (b) the developmental patterns of mice obtained from embryos cultured in the presence or absence of myo-Ins, up to three weeks of age, overlap. These data further identify myo-Ins as a possibly important supplement for human preimplantation embryo culture in assisted reproduction technology.
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影响因子：
16.2
作者：
D'Anna R;Scilipoti A;Giordano D;Caruso C;Cannata ML;Interdonato ML;Corrado F;Di Benedetto A
通讯作者：
Di Benedetto A
影响因子：
2
作者：
Bevilacqua, Arturo;Carlomagno, Gianfranco;Kamenov, Zdravko A.
通讯作者：
Kamenov, Zdravko A.
影响因子：
2
作者：
D'Anna, R.;Di Benedetto, V.;Di Benedetto, A.
通讯作者：
Di Benedetto, A.
影响因子：
6.1
作者：
Chiu, TTY;Rogers, MS;Haines, CJ
通讯作者：
Haines, CJ
影响因子：
7.5
作者：
Downward, J
通讯作者：
Downward, J