Whole-Body Single-Cell Sequencing Reveals Transcriptional Domains in the Annelid Larval Body.
Whole-Body Single-Cell Sequencing Reveals Transcriptional Domains in the Annelid Larval Body.
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DOI：
10.1093/molbev/msx336
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发表时间：
2018-05-01
影响因子：
10.7
通讯作者：
Arendt D
中科院分区：
文献类型：
--
作者：
Achim K;Eling N;Vergara HM;Bertucci PY;Musser J;Vopalensky P;Brunet T;Collier P;Benes V;Marioni JC;Arendt D
动物身体由各种各样的细胞组成。为了表征整个身体的细胞身份，我们比较了从海洋环节动物Platynereis dumerilii的解离全幼虫中随机挑选的单细胞的转录组。我们确定了五个转录不同的分化细胞组，每个表达一组独特的转录因子和效应基因，实现细胞表型。空间映射到细胞表达图谱的细胞，和组特异性基因的wholemount原位杂交揭示了空间连贯的转录结构域在幼虫体内，包括，例如，顶端感觉神经分泌细胞与神经/表皮表面细胞。这些领域代表了新的，基本的细分环节动物体完全基于差异基因表达，并组成了多个，转录相似的细胞类型。它们不代表克隆域，如发育谱系分析所示。我们建议，细分环节动物幼虫体的转录结构域代表了进化多样化所产生的相关细胞类型的家庭。它们可能的进化保守性使它们成为进化发育研究的一个有前途的工具。
Animal bodies comprise diverse arrays of cells. To characterize cellular identities across an entire body, we have compared the transcriptomes of single cells randomly picked from dissociated whole larvae of the marine annelid Platynereis dumerilii. We identify five transcriptionally distinct groups of differentiated cells, each expressing a unique set of transcription factors and effector genes that implement cellular phenotypes. Spatial mapping of cells into a cellular expression atlas, and wholemount in situ hybridization of group-specific genes reveals spatially coherent transcriptional domains in the larval body, comprising, for example, apical sensory-neurosecretory cells versus neural/epidermal surface cells. These domains represent new, basic subdivisions of the annelid body based entirely on differential gene expression, and are composed of multiple, transcriptionally similar cell types. They do not represent clonal domains, as revealed by developmental lineage analysis. We propose that the transcriptional domains that subdivide the annelid larval body represent families of related cell types that have arisen by evolutionary diversification. Their possible evolutionary conservation makes them a promising tool for evo–devo research.
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影响因子：
2.7
作者：
Bates, Karen E.;Whitington, Paul M.
通讯作者：
Whitington, Paul M.
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
56.9
作者：
Arendt, D;Tessmar-Raible, K;Wittbrodt, J
通讯作者：
Wittbrodt, J
影响因子：
64.5
作者：
Denes, Alexandru S.;Jekely, Gaspar;Arendt, Detlev
通讯作者：
Arendt, Detlev
DOI：
10.1146/annurev-vision-090814-121657
发表时间：
2015-01-01
期刊：
ANNUAL REVIEW OF VISION SCIENCE, VOL 1
影响因子：
--
作者：
Cepko, Constance L.
通讯作者：
Cepko, Constance L.