The photomorphogenic factors UV-B RECEPTOR 1, ELONGATED HYPOCOTYL 5, and HY5 HOMOLOGUE are part of the UV-B signalling pathway in grapevine and mediate flavonol accumulation in response to the environment.
The photomorphogenic factors UV-B RECEPTOR 1, ELONGATED HYPOCOTYL 5, and HY5 HOMOLOGUE are part of the UV-B signalling pathway in grapevine and mediate flavonol accumulation in response to the environment.
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DOI：
10.1093/jxb/erw307
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发表时间：
2016-10
影响因子：
6.9
通讯作者：
Arce-Johnson P
中科院分区：
文献类型：
--
作者：
Loyola R;Herrera D;Mas A;Wong DC;Höll J;Cavallini E;Amato A;Azuma A;Ziegler T;Aquea F;Castellarin SD;Bogs J;Tornielli GB;Peña-Neira A;Czemmel S;Alcalde JA;Matus JT;Arce-Johnson P
通过结合辐射实验和计算机系统分析进行分子研究，我们确定了葡萄 UV-B 受体和两种 HY5 同系物在调节黄酮醇合成中的作用。葡萄树 (Vitis vinifera L.) 是一种因其对辐射的适应能力而闻名的物种。然而，与 UV-B 反应相关的光形态形成因子尚未得到分子表征。我们从葡萄中克隆并研究了 UV-B 受体 (UVR1)、伸长的下胚轴 5 (HY5) 和 HY5 同源物 (HYH) 的作用。我们进行了基因功能表征，生成了共表达网络，并在不同的环境条件下对其进行了测试。这些基因补充了拟南芥 uvr8 和 hy5 突变体对辐射的形态和次级代谢反应。我们将微阵列和 RNA 测序 (RNA-seq) 数据与启动子检查相结合，以确定 HY5 和 HYH 假定目标基因及其 DNA 结合偏好。尽管共享大量共同表达的基因，但我们发现 HY5 和 HYH 根据器官和应激条件具有不同的层次结构，反映了合作和部分冗余的角色。 HY5 结合位点的存在支持了新的候选 UV-B 基因标记。其中包括一组黄酮醇相关基因，这些基因在 HY5 瞬时表达测定中上调。我们用高和低的 UV-B 照射对离体盆栽藤本植物的幼苗和果实进行了照射，并与未照射条件相比跟踪了黄酮醇的积累和基因表达的变化。 UVR1、HY5 和 HYH 表达随器官、发育阶段和辐射类型的不同而变化。令人惊讶的是，浆果中的 UVR1 表达受到遮荫和温度的调节，但不受 UV-B 辐射的调节。我们提出，UV-B 响应机制通过在高和低 UV-B 暴露的不同发育阶段激活 HY5 和 HYH，有利于浆果黄酮醇的积累。
By performing molecular studies coupled to radiation experiments and in silico systems analyses, we have ascertained the role of the grapevine UV-B receptor and two HY5 homologues in regulating flavonol synthesis. Grapevine (Vitis vinifera L.) is a species well known for its adaptation to radiation. However, photomorphogenic factors related to UV-B responses have not been molecularly characterized. We cloned and studied the role of UV-B RECEPTOR (UVR1), ELONGATED HYPOCOTYL 5 (HY5), and HY5 HOMOLOGUE (HYH) from V. vinifera. We performed gene functional characterizations, generated co-expression networks, and tested them in different environmental conditions. These genes complemented the Arabidopsis uvr8 and hy5 mutants in morphological and secondary metabolic responses to radiation. We combined microarray and RNA sequencing (RNA-seq) data with promoter inspections to identify HY5 and HYH putative target genes and their DNA binding preferences. Despite sharing a large set of common co-expressed genes, we found different hierarchies for HY5 and HYH depending on the organ and stress condition, reflecting both co-operative and partially redundant roles. New candidate UV-B gene markers were supported by the presence of HY5-binding sites. These included a set of flavonol-related genes that were up-regulated in a HY5 transient expression assay. We irradiated in vitro plantlets and fruits from old potted vines with high and low UV-B exposures and followed the accumulation of flavonols and changes in gene expression in comparison with non-irradiated conditions. UVR1, HY5, and HYH expression varied with organ, developmental stage, and type of radiation. Surprisingly, UVR1 expression was modulated by shading and temperature in berries, but not by UV-B radiation. We propose that the UV-B response machinery favours berry flavonol accumulation through the activation of HY5 and HYH at different developmental stages at both high and low UV-B exposures.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
1.2
作者：
Azuma, Akifumi;Fujii, Hiroshi;Yakushiji, Hiroshi
通讯作者：
Yakushiji, Hiroshi
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
64.8
作者：
Jaillon, Olivier;Aury, Jean-Marc;Wincker, Patrick
通讯作者：
Wincker, Patrick
影响因子：
7.4
作者：
Cavallini, Erika;Tomas Matus, Jose;Tornielli, Giovanni Battista
通讯作者：
Tornielli, Giovanni Battista