Studies on Xenopus laevis intestine reveal biological pathways underlying vertebrate gut adaptation from embryo to adult.
Studies on Xenopus laevis intestine reveal biological pathways underlying vertebrate gut adaptation from embryo to adult.
复制标题
DOI：
10.1186/gb-2010-11-5-r55
复制
发表时间：
2010
期刊：
影响因子：
12.3
通讯作者：
Shi YB
中科院分区：
文献类型：
--
作者：
Heimeier RA;Das B;Buchholz DR;Fiorentino M;Shi YB
非洲爪哇从胚胎到成体肠道的发育转录组揭示了所有脊椎动物肠道发育的规律。为了适应不断变化的饮食环境，在脊椎动物的发育过程中，从胚胎到成年，消化道都进行了广泛的重塑。非洲爪哇变态是研究哺乳动物胃肠道发育的一个很好的模型系统，用于确定肠道发育和成熟所必需的基因和信号程序。变态肠道可分为四个不同的发育时间点，并用莱维氏X.laevis基因芯片进行分析。由于发育信号程序的高度保守性和与哺乳动物基因的同源性，注释和生物信息学分析都是基于人类的同源基因。表达模式的聚集揭示了共同表达的基因参与了必要的细胞过程，如凋亡和增殖。两个最大的基因簇在变态的高潮时分别有表达高峰和表达低谷。在此期间调节的新的保守基因本体论类别包括转录活性、信号转导和代谢过程。此外，我们还鉴定了幼虫/胚胎和成虫特有的基因。详细分析发现了17个幼虫特异基因，这些基因可能代表人类结肠癌的分子标记，而许多成人特异基因与饮食酶有关。这项全球发育表达研究首次提供了胚胎后发育过程中肠道重塑和成熟的详细分子描述，这将有助于我们更好地理解肠道器官发生和人类疾病。这项研究有助于我们理解发育和组织更新过程中分子调控的动力学，这对未来的基础和临床研究以及医学应用具有重要意义。
The developmental transcriptome of the Xenopus laevis intestine, from embryo to adult, reveals insights into the regulation of gut development in all vertebrates. To adapt to its changing dietary environment, the digestive tract is extensively remodeled from the embryo to the adult during vertebrate development. Xenopus laevis metamorphosis is an excellent model system for studying mammalian gastrointestinal development and is used to determine the genes and signaling programs essential for intestinal development and maturation. The metamorphosing intestine can be divided into four distinct developmental time points and these were analyzed with X. laevis microarrays. Due to the high level of conservation in developmental signaling programs and homology to mammalian genes, annotations and bioinformatics analysis were based on human orthologs. Clustering of the expression patterns revealed co-expressed genes involved in essential cell processes such as apoptosis and proliferation. The two largest clusters of genes have expression peaks and troughs at the climax of metamorphosis, respectively. Novel conserved gene ontology categories regulated during this period include transcriptional activity, signal transduction, and metabolic processes. Additionally, we identified larval/embryo- and adult-specific genes. Detailed analysis revealed 17 larval specific genes that may represent molecular markers for human colonic cancers, while many adult specific genes are associated with dietary enzymes. This global developmental expression study provides the first detailed molecular description of intestinal remodeling and maturation during postembryonic development, which should help improve our understanding of intestinal organogenesis and human diseases. This study significantly contributes towards our understanding of the dynamics of molecular regulation during development and tissue renewal, which is important for future basic and clinical research and for medicinal applications.
登录
查看更多内容
影响因子：
56.9
作者：
NEURATH, H
通讯作者：
NEURATH, H
影响因子：
64.5
作者：
Liu, XS;Zou, H;Wang, XD
通讯作者：
Wang, XD
影响因子：
5.3
作者：
Muraoka, RS;Koh, Y;Arteaga, CL
通讯作者：
Arteaga, CL
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.6
作者：
Hasebe, T;Hartman, R;Shi, YB
通讯作者：
Shi, YB