A microsatellite-based consensus linkage map for species of Eucalyptus and a novel set of 230 microsatellite markers for the genus.
A microsatellite-based consensus linkage map for species of Eucalyptus and a novel set of 230 microsatellite markers for the genus.
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DOI：
10.1186/1471-2229-6-20
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发表时间：
2006-09-22
影响因子：
5.3
通讯作者：
Grattapaglia D
中科院分区：
文献类型：
--
作者：
Brondani RP;Williams ER;Brondani C;Grattapaglia D
桉树是世界上种植最广泛的硬木树种，全球种植面积超过1800万公顷，是碳中性可再生能源的重要来源，也是纸浆、纸张和实木的原料。桉树的数量性状基因座(QTL)已被定位在系谱特异的RAPD或AFLP图谱上，严重限制了这种QTL定位在分子育种中的价值。获得具有可转移微卫星标记的全属遗传图谱已成为有效推进基因组事业的必要条件。这份报告描述了一组新的230个Embra微卫星的开发，构建了第一个全面的基于微卫星的桉树共识连锁图谱，并整合了现有的其他微卫星和定位于该属其他物种的候选基因的连锁信息。该图谱覆盖了桉树约90%的重组基因组，涉及11个连锁群上的234个Embra基因座，观察长度为1,568 cM，标记间平均距离为8.4 cM。在桉树上发表的所有微卫星连锁信息的汇编使我们能够在这一共识图谱与为蓝鳍金龟子出版的其他图谱之间建立连锁群之间的同源性。通过比较作图分析，还确定了来自其他桉树种的另外41个微卫星的连锁群，以及文献中发表的木材和开花性状的候选基因和QTL。这份报告大大增加了桉树属物种微卫星标记和作图信息的可用性，并证实了该属物种之间微卫星侧翼序列和基因座序列的高度保守性。这项工作代表了桉树比较基因组学向前迈出的重要一步，为进化研究和分子育种应用开辟了令人振奋的前景。广泛使用越来越多的种间可转移标记和共识作图信息，将允许更快和更详细地研究物种间的QTL共性，验证不同遗传背景下的表达-QTL，并定位越来越多与QTL共定位的候选基因，以便在关联作图实验中进行测试。
Eucalypts are the most widely planted hardwood trees in the world occupying globally more than 18 million hectares as an important source of carbon neutral renewable energy and raw material for pulp, paper and solid wood. Quantitative Trait Loci (QTLs) in Eucalyptus have been localized on pedigree-specific RAPD or AFLP maps seriously limiting the value of such QTL mapping efforts for molecular breeding. The availability of a genus-wide genetic map with transferable microsatellite markers has become a must for the effective advancement of genomic undertakings. This report describes the development of a novel set of 230 EMBRA microsatellites, the construction of the first comprehensive microsatellite-based consensus linkage map for Eucalyptus and the consolidation of existing linkage information for other microsatellites and candidate genes mapped in other species of the genus. The consensus map covers ~90% of the recombining genome of Eucalyptus, involves 234 mapped EMBRA loci on 11 linkage groups, an observed length of 1,568 cM and a mean distance between markers of 8.4 cM. A compilation of all microsatellite linkage information published in Eucalyptus allowed us to establish the homology among linkage groups between this consensus map and other maps published for E. globulus. Comparative mapping analyses also resulted in the linkage group assignment of other 41 microsatellites derived from other Eucalyptus species as well as candidate genes and QTLs for wood and flowering traits published in the literature. This report significantly increases the availability of microsatellite markers and mapping information for species of Eucalyptus and corroborates the high conservation of microsatellite flanking sequences and locus ordering between species of the genus. This work represents an important step forward for Eucalyptus comparative genomics, opening stimulating perspectives for evolutionary studies and molecular breeding applications. The generalized use of an increasingly larger set of interspecific transferable markers and consensus mapping information, will allow faster and more detailed investigations of QTL synteny among species, validation of expression-QTL across variable genetic backgrounds and positioning of a growing number of candidate genes co-localized with QTLs, to be tested in association mapping experiments.
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影响因子：
3.1
作者：
Brondani, RPV;Brondani, C;Grattapaglia, D
通讯作者：
Grattapaglia, D
影响因子：
5.4
作者：
Byrne, M;Murrell, JC;Moran, GF
通讯作者：
Moran, GF
影响因子：
5.4
作者：
BYRNE, M;MURRELL, JC;MORAN, GF
通讯作者：
MORAN, GF
影响因子：
5.4
作者：
DEVEY, ME;FIDDLER, TA;NEALE, DB
通讯作者：
NEALE, DB
影响因子：
3.1
作者：
Glaubitz, JC;Emebiri, LC;Moran, GF
通讯作者：
Moran, GF