Pea PSII-LHCII supercomplexes form pairs by making connections across the stromal gap.
Pea PSII-LHCII supercomplexes form pairs by making connections across the stromal gap.
复制标题
PEA PSII-LHCII超复合物通过在基质间隙之间建立连接来形成对。
DOI：
10.1038/s41598-017-10700-8
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发表时间：
2017-08-30
影响因子：
4.6
通讯作者：
Pagliano C
中科院分区：
文献类型：
--
作者：
Albanese P;Melero R;Engel BD;Grinzato A;Berto P;Manfredi M;Chiodoni A;Vargas J;Sorzano CÓS;Marengo E;Saracco G;Zanotti G;Carazo JM;Pagliano C
在高等植物类囊体中，光合蛋白复合物的不均匀分布是形成基粒的决定因素，基粒是密集分布有光系统II（PSII）及其捕光复合物（LHCII）的膜盘堆叠。PSII与LHCII结合形成PSII-LHCII超复合体，这是太阳能转换的关键组成部分。在这里，我们报告的PSII-LHCII超复合物，这是在生理相关的阳离子浓度下分离的对的生化，结构和功能特性。使用单粒子冷冻电子显微镜，我们确定了配对的C2 S2 M PSII-LHCII超复合物的三维结构，分辨率为14 μ m。这两个超复合物通过并列LHCII三聚体之间的特定重叠和跨越基质间隙的物理连接在其基质侧相互作用，其中一个可能是由两个Lhcb 4单体LHCII亚基的N-末端环之间的相互作用形成的。快速叶绿素荧光诱导分析表明，配对PSII-LHCII超复合物的能量耦合。分子动力学模拟表明，额外的灵活的物理连接之间可能形成并列LHCII三聚体的配对PSII-LHCII超复合物贴压类囊体膜。我们的研究结果提供了新的见解PSII-LHCII超复合物对之间的相互作用可以连接相邻的类囊体介导的基粒膜的堆叠。
In higher plant thylakoids, the heterogeneous distribution of photosynthetic protein complexes is a determinant for the formation of grana, stacks of membrane discs that are densely populated with Photosystem II (PSII) and its light harvesting complex (LHCII). PSII associates with LHCII to form the PSII-LHCII supercomplex, a crucial component for solar energy conversion. Here, we report a biochemical, structural and functional characterization of pairs of PSII-LHCII supercomplexes, which were isolated under physiologically-relevant cation concentrations. Using single-particle cryo-electron microscopy, we determined the three-dimensional structure of paired C2S2M PSII-LHCII supercomplexes at 14 Å resolution. The two supercomplexes interact on their stromal sides through a specific overlap between apposing LHCII trimers and via physical connections that span the stromal gap, one of which is likely formed by interactions between the N-terminal loops of two Lhcb4 monomeric LHCII subunits. Fast chlorophyll fluorescence induction analysis showed that paired PSII-LHCII supercomplexes are energetically coupled. Molecular dynamics simulations revealed that additional flexible physical connections may form between the apposing LHCII trimers of paired PSII-LHCII supercomplexes in appressed thylakoid membranes. Our findings provide new insights into how interactions between pairs of PSII-LHCII supercomplexes can link adjacent thylakoids to mediate the stacking of grana membranes.
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影响因子：
3
作者：
de la Rosa-Trevin, J. M.;Quintana, A.;Carazo, J. M.
通讯作者：
Carazo, J. M.
影响因子：
4.3
作者：
Albanese, Pascal;Manfredi, Marcello;Pagliano, Cristina
通讯作者：
Pagliano, Cristina
影响因子：
3.7
作者：
Albanese, Pascal;Nield, Jon;Pagliano, Cristina
通讯作者：
Pagliano, Cristina
影响因子：
11.6
作者：
Daum, Bertram;Nicastro, Daniela;Kuehlbrandt, Werner
通讯作者：
Kuehlbrandt, Werner
影响因子：
7.2
作者：
Allahverdiyeva, Yagut;Suorsa, Marjaana;Pesaresi, Paolo
通讯作者：
Pesaresi, Paolo