Association study of wheat grain protein composition reveals that gliadin and glutenin composition are trans-regulated by different chromosome regions.
Association study of wheat grain protein composition reveals that gliadin and glutenin composition are trans-regulated by different chromosome regions.
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DOI：
10.1093/jxb/ert188
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发表时间：
2013-09
影响因子：
6.9
通讯作者：
Martre P
中科院分区：
文献类型：
--
作者：
Plessis A;Ravel C;Bordes J;Balfourier F;Martre P
小麦籽粒贮藏蛋白（GSP）含量和组成是决定面包小麦（Triticum aestivum L.）粮食麦谷蛋白和醇溶蛋白是小麦中两类主要的GSP，其积累主要通过转录因子网络在转录水平上控制。这一调控网络可以导致稳定的跨环境的异速生长的比例关系的GSP类/亚基的数量和每粒总氮量。本工作进行了遗传作图研究的GSP含量和组成和异速生长的比例参数的粮食氮分配使用面包小麦全球核心收集在三个环境中生长。对核心样本进行了基因分型，其中873个标记用于全基因组关联，167个单核苷酸多态性标记用于51个候选基因的候选关联。候选基因包括35个在籽粒中表达的转录因子。这项工作确定了74个位点与38个变量，其中19个是候选基因或与候选基因紧密连锁。除了结构性GSP基因外，还鉴定了几个推定反式调节GSP积累的基因座。7个候选TF，包括4个控制大麦醇溶蛋白基因表达的小麦直向同源物TF，与麦谷蛋白或醇溶蛋白的组成或数量，或异速生长谷物N分配参数相关的标记相关或处于强连锁不平衡，证实了GSP积累的转录控制的重要性。全基因组关联的结果表明，调节麦谷蛋白和醇溶蛋白组成的基因大多是彼此不同的和不同的操作。
Wheat grain storage protein (GSP) content and composition are the main determinants of the end-use value of bread wheat (Triticum aestivum L.) grain. The accumulation of glutenins and gliadins, the two main classes of GSP in wheat, is believed to be mainly controlled at the transcriptional level through a network of transcription factors. This regulation network could lead to stable cross-environment allometric scaling relationships between the quantity of GSP classes/subunits and the total quantity of nitrogen per grain. This work conducted a genetic mapping study of GSP content and composition and allometric scaling parameters of grain N allocation using a bread wheat worldwide core collection grown in three environments. The core collection was genotyped with 873 markers for genome-wide association and 167 single nucleotide polymorphism markers in 51 candidate genes for candidate association. The candidate genes included 35 transcription factors (TFs) expressed in grain. This work identified 74 loci associated with 38 variables, of which 19 were candidate genes or were tightly linked with candidate genes. Besides structural GSP genes, several loci putatively trans-regulating GSP accumulation were identified. Seven candidate TFs, including four wheat orthologues of barley TFs that control hordein gene expression, were associated or in strong linkage disequilibrium with markers associated with the composition or quantity of glutenin or gliadin, or allometric grain N allocation parameters, confirming the importance of the transcriptional control of GSP accumulation. Genome-wide association results suggest that the genes regulating glutenin and gliadin compositions are mostly distinct from each other and operate differently.
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影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
5.1
作者：
Dong, Guoqing;Ni, Zhongfu;Sun, Qixin
通讯作者：
Sun, Qixin
影响因子：
3.8
作者：
Fu, BX;Kovacs, MIP
通讯作者：
Kovacs, MIP
影响因子：
3.7
作者：
Dong L;Zhang X;Liu D;Fan H;Sun J;Zhang Z;Qin H;Li B;Hao S;Li Z;Wang D;Zhang A;Ling HQ
通讯作者：
Ling HQ
影响因子：
5.4
作者：
Balfourier, Francois;Roussel, Valerie;Charmet, Gilles
通讯作者：
Charmet, Gilles