Interpenetrating fibrin-alginate matrices for in vitro ovarian follicle development.
Interpenetrating fibrin-alginate matrices for in vitro ovarian follicle development.
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DOI：
10.1016/j.biomaterials.2009.06.054
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发表时间：
2009-10
期刊：
影响因子：
14
通讯作者：
Shea, Lonnie D.
中科院分区：
文献类型：
--
作者：
Shikanov, Ariella;Xu, Min;Woodruff, Teresa K.;Shea, Lonnie D.
关键词：
在本报告中，我们研究了纤维蛋白-海藻酸盐互穿网络（FA-IPN）提供动态细胞响应力学特性，并将其应用于卵巢卵泡的体外生长。用流变学方法研究了凝胶的力学性能和聚合速率，并用电子显微镜对纤维结构进行了成像。采用小鼠模型，将两层次卵泡包埋在FA-IPNs中，并评估其生长、形态、激素产生、纤维蛋白降解率和能态卵的数量。FA-IPN的初始力学是由复合材料决定的，随后被包裹的细胞对纤维蛋白的降解会产生一种由于海藻酸盐而具有机械性能的材料。FA-IPN培养的卵母细胞具有同种性的率为82%，显著高于单独海藻酸盐培养。卵母细胞质量的提高是确定3D培养系统的重要一步，可以为研究卵泡成熟提供基本工具，并可用于促进人类卵泡的生长，这可用于为面临癌症诊断的女性提供生殖选择
In this report, we investigate the fibrin-alginate inter penetrating network (FA-IPN) to provide dynamic cell-responsive mechanical properties, which we apply to the in vitro growth of ovarian follicles. The mechanical properties and polymerization rate of the gels were investigated by rheology, and the fiber structure was imaged by electron microscopy. Using a mouse model, two-layered secondary follicles were encapsulated in FA-IPNs, and growth, morphology, hormone production, fibrin degradation rate and the numbers of competent eggs were assessed. The initial mechanics of the FA-IPN are determined by the composite material, and subsequent degradation of fibrin by the encapsulated cells would produce a material with mechanical properties due to the alginate alone. The rate of meotically competent oocytes produced by culture in FA-IPN was 82%, which was significantly greater than in alginate alone. This increase in oocyte quality is an important step in identifying 3D culture systems that can provide a fundamental tool to investigate follicle maturation, and may be applied to promote the growth of human follicles, which can be used to provide reproductive options for women facing a cancer diagnosis
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影响因子：
3.8
作者：
Xu, Min;Banc, Anna;Woodruff, Teresa K.;Shea, Lonnie D.
通讯作者：
Shea, Lonnie D.
影响因子：
3.8
作者：
Discher, Dennis;Dong, Cheng;Fredberg, Jeffrey J.;Guilak, Farshid;Ingber, Donald;Janmey, Paul;Kamm, Roger D.;Schmid-Schoenbein, Geert W.;Weinbaum, Sheldon
通讯作者：
Weinbaum, Sheldon
影响因子：
3.6
作者：
West-Farrell ER;Xu M;Gomberg MA;Chow YH;Woodruff TK;Shea LD
通讯作者：
Shea LD
影响因子：
4
作者：
Bao, Lei;Yang, Wei;Tang, Shunqing
通讯作者：
Tang, Shunqing
影响因子：
3.6
作者：
Xu, Min;West, Erin;Woodruff, Teresa K.
通讯作者：
Woodruff, Teresa K.