Secondary follicle growth and oocyte maturation by culture in alginate hydrogel following cryopreservation of the ovary or individual follicles.
Secondary follicle growth and oocyte maturation by culture in alginate hydrogel following cryopreservation of the ovary or individual follicles.
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DOI：
10.1002/bit.22250
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发表时间：
2009-06-01
影响因子：
3.8
通讯作者：
Shea, Lonnie D.
中科院分区：
文献类型：
--
作者：
Xu, Min;Banc, Anna;Woodruff, Teresa K.;Shea, Lonnie D.
对于面临癌症诊断的女性，生育力保存的一种选择涉及冷冻保存卵巢组织以供以后再次移植或体外培养，优选体外培养以避免再次引入癌症。小的、未成熟的卵泡在冻融过程中存活下来，并且可以通过卵泡内成熟（IFM）而成熟，所述卵泡内成熟包括卵泡的初始生长和随后的卵母细胞的成熟。卵巢组织可以两种形式冷冻保存：（i）由卵泡和周围基质组成的皮质条（Cryo-Ov）或（ii）单独分离的卵泡（Cryo-In）。本研究的目的是评估卵泡生长和卵母细胞成熟的卵泡，冷冻保存无论是作为条或单独使用缓慢冷冻冷冻保存方法。两个卵泡组与非冷冻保存的对照卵泡一起在基于藻酸盐的三维培养系统中生长12天。三组的总生存率、体积增加率和胃窦形成率相当。在第12天的文化，雄烯二酮水平下降的Cryo-Ov组相对于其他两个，和孕酮雌二醇的比例增加的两个冷冻保存组相对于对照组。Gja 1（称为连接蛋白43）和Gja 4（称为连接蛋白37）mRNA表达在第6天在冷冻保存组相对于对照组降低，到第12天，Gja 1在所有3组中相似。此外，Cryo-In导致较低的GVBD率，表明卵母细胞发育受损。总体而言，本研究表明，小鼠腔前卵泡，无论是在卵巢组织或单独分离，可以成功地冷冻保存的缓慢冷冻方法，证明解冻后卵泡发育和类固醇生成，卵母细胞成熟和卵母细胞和/或颗粒细胞连接的分子标记。
An option for fertility preservation for women facing a cancer diagnosis involves the cryopreservation of ovarian tissue for later re-transplantation or in vitro culture, with in vitro culture preferred to avoid reintroduction of the cancer. Small, immature follicles survive the freeze-thaw process, and can be matured through in follicle maturation (IFM) that involves an initial growth of the follicle and subsequent maturation of the oocyte. The ovarian tissue can be cryopreserved in two forms: (i) cortical strips consisting of follicles and surrounding stroma (Cryo-Ov) or (ii) individually isolated follicles (Cryo-In). The aim of this study was to assess the follicle growth and oocyte maturation for follicles that were cryopreserved either as strips or individually using a slow-freezing cryopreservation method. The two follicle groups, together with non-cryopreserved control follicles, were grown in an alginate-based three-dimensional culture system for twelve days. The overall survival, size increase and antrum formation rates were comparable among the three groups. At day 12 of culture, Androstenedione levels were decreased in the Cryo-Ov group relative to the other two, and the ratio of progesterone to estradiol was increased in the two cryopreserved groups relative to the control. Both Gja1 (known as connexin 43) and Gja4 (known as connexin 37) mRNA expression were decreased at day 6 in the cryopreserved groups relative to controls, and by day 12, Gja1 was similar for all 3 groups. Moreover, Cryo-In resulted in lower GVBD rate indicating some impaired oocyte development. Overall, the present study demonstrated that mouse preantral follicles, either within ovarian tissues or individually isolated, could be successfully cryopreserved by the slow-freezing method, as evidenced by post-thaw follicle development and steroidgenesis, oocyte maturation and molecular markers for oocyte and/or granulosa cells connection.
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影响因子：
4
作者：
Gittens, JEI;Kidder, GM
通讯作者：
Kidder, GM
影响因子：
4
作者：
Gittens, JEI;Barr, KJ;Kidder, GM
通讯作者：
Kidder, GM
影响因子：
3.8
作者：
Hani, T;Tachibe, T;Jishage, K
通讯作者：
Jishage, K
影响因子：
6.1
作者：
Gunasena, KT;Villines, PM;Critser, JK
通讯作者：
Critser, JK
影响因子：
6.1
作者：
CARROLL, J;GOSDEN, RG
通讯作者：
GOSDEN, RG