In vivo magnetic enrichment and multiplex photoacoustic detection of circulating tumour cells.
In vivo magnetic enrichment and multiplex photoacoustic detection of circulating tumour cells.
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DOI：
10.1038/nnano.2009.333
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发表时间：
2009-12
影响因子：
38.3
通讯作者：
中科院分区：
文献类型：
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作者：
癌细胞在器官之间扩散，这一过程被称为转移，是大多数癌症死亡的原因。检测循环肿瘤细胞（一种常见的转移标志物）是很困难的，因为体外方法由于血液样本量有限而不够敏感，而体内诊断则耗时，因为必须分析大量血液。在这里，我们展示了一种通过快速光声检测在小鼠血液中磁捕获循环肿瘤细胞的方法。磁性纳米颗粒被功能化，以乳腺癌细胞中常见的受体为目标，在磁铁下结合并捕获循环的肿瘤细胞。为了提高光声成像的灵敏度和特异性，我们将叶酸偶联的镀金碳纳米管作为光声成像的第二造影剂。通过整合体内多重靶向、磁富集、信号放大和多色识别，我们的方法可以从载瘤小鼠血管中的大量血液中浓缩循环肿瘤细胞，这可能具有早期诊断癌症和预防人类转移的潜力。
The spread of cancer cells between organs, a process known as metastasis, is the cause of most cancer deaths. Detecting circulating tumour cells—a common marker for the development of metastasis—is difficult because ex vivo methods are not sensitive enough owing to limited blood sample volume and in vivo diagnosis is time-consuming as large volumes of blood must be analysed. Here, we show a way to magnetically capture circulating tumour cells in the bloodstream of mice followed by rapid photoacoustic detection. Magnetic nanoparticles, which were functionalized to target a receptor commonly found in breast cancer cells, bound and captured circulating tumour cells under a magnet. To improve detection sensitivity and specificity, gold-plated carbon nanotubes conjugated with folic acid were used as a second contrast agent for photoacoustic imaging. By integrating in vivo multiplex targeting, magnetic enrichment, signal amplification and multicolour recognition, our approach allows circulating tumour cells to be concentrated from a large volume of blood in the vessels of tumour-bearing mice, and this could have potential for the early diagnosis of cancer and the prevention of metastasis in humans.
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影响因子：
3.5
作者：
Larson, Timothy A.;Bankson, James;Sokolov, Konstantin
通讯作者：
Sokolov, Konstantin
影响因子：
3.7
作者：
Galanzha, Ekaterina I.;Shashkov, Evgeny V.;Tuchin, Valery V.;Zharov, Vladimir P.
通讯作者：
Zharov, Vladimir P.
影响因子：
38.3
作者：
通讯作者：
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影响因子：
3.5
作者：
Ermilov, Sergey A.;Khamapirad, Tuenchit;Oraevsky, Alexander A.
通讯作者：
Oraevsky, Alexander A.
DOI：
10.1158/1078-0432.ccr-08-3289
发表时间：
2009-07-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Yang L;Peng XH;Wang YA;Wang X;Cao Z;Ni C;Karna P;Zhang X;Wood WC;Gao X;Nie S;Mao H
通讯作者：
Mao H