Preparation and evaluation of parathyroid hormone incorporated CaP coating via a biomimetic method.
Preparation and evaluation of parathyroid hormone incorporated CaP coating via a biomimetic method.
复制标题
仿生方法制备并评价甲状旁腺激素掺入 CaP 涂层。
DOI：
10.1002/jbm.b.31820
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
["Xiaohua Yu
中科院分区：
文献类型：
--
作者：
["Xiaohua Yu
甲状旁腺激素（PTH）是一种有效的骨生长刺激剂，用于治疗骨质疏松症。然而，日常给药的不便和全身暴露的副作用严重限制了其在临床上的应用。局部控制给药是一种很有前途的方法，它可以在局部长时间维持治疗浓度。在这项研究中，甲状旁腺素被纳入仿生磷酸钙（CaP）涂层通过共沉淀过程中的修改模拟体液（m-SBF）。结果表明，PTH成功地结合到钛表面的仿生CaP涂层，具有较高的结合效率。PTH的加入显著改变了涂层的形貌，但涂层的组成保持不变。PTH在体外有局部释放，并伴有CaP涂层的部分溶解。细胞培养实验表明，钙磷涂层释放的PTH完全保留了其生物活性。显著促进MC 3 T3-E1细胞增殖，但轻微延迟细胞碱性磷酸酶（ALP）的表达。总之，我们的研究结果表明，钙磷涂层结合PTH可能是一个有前途的方法，骨质疏松症和其他骨相关疾病的治疗在未来。
Parathyroid hormone (PTH) is a potent bone growth stimulator used for osteoporosis treatment. However, the inconvenience of daily administration and side effect of systemic exposure severely limit its use in clinical applications. Local, controlled delivery is a promising approach which can maintain therapeutic concentration locally for a long period. In this study, PTH was incorporated into a biomimetic calcium phosphate (CaP) coating via a coprecipitation process in a modified simulated body fluid (m-SBF). It was found that PTH was successfully incorporated into biomimetic CaP coating on titanium surface with a high incorporation efficiency. The incorporation of PTH into coatings had significantly changed the coating morphology, but the composition of the coating remained unchanged. Localized release of PTH had occurred in vitro, and was accompanied with partial dissolution of CaP coatings. Cell culture study demonstrated that the PTH released from CaP coatings fully retained its bioactivity. It had improved substantially MC3T3-E1 cell proliferation but slightly delayed the expression of alkaline phosphatase (ALP) of the cells. In summary, our results have shown that CaP coatings incorporated with PTH may be a promising approach for osteoporosis and other bone-related disease treatment in the future.
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影响因子：
4.1
作者：
Jilka, Robert L.;O'Brien, Charles A.;Ali, A. Afshan;Roberson, Paula K.;Weinstein, Robert S.;Manolagas, Stavros C.
通讯作者：
Manolagas, Stavros C.
影响因子：
14
作者：
Murphy, WL;Hsiong, S;Mooney, DJ
通讯作者：
Mooney, DJ
影响因子：
14
作者：
Murphy, WL;Peters, MC;Mooney, DJ
通讯作者：
Mooney, DJ
DOI：
10.1073/pnas.93.12.5753
发表时间：
1996-06-11
影响因子：
11.1
作者：
Fang, JM;Zhu, YY;Roessler, BJ
通讯作者：
Roessler, BJ
影响因子：
14
作者：
Chou, YF;Chiou, WA;Wu, BM
通讯作者：
Wu, BM