The use of sodium trimetaphosphate as a biomimetic analog of matrix phosphoproteins for remineralization of artificial caries-like dentin.
The use of sodium trimetaphosphate as a biomimetic analog of matrix phosphoproteins for remineralization of artificial caries-like dentin.
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DOI：
10.1016/j.dental.2011.01.008
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发表时间：
2011-05
期刊：
影响因子：
5
通讯作者：
Tay, Franklin R.
中科院分区：
文献类型：
--
作者：
Liu, Yan;Li, Nan;Qi, Yipin;Niu, Li-na;Elshafiy, Sally;Mao, Jing;Breschi, Lorenzo;Pashley, David H.;Tay, Franklin R.
这项研究考察了使用三偏磷酸钠(STMP)作为基质磷蛋白的仿生类似物来重新矿化人工龋齿影响的牙本质。人工龋病灶深度300±30微米，采用pH循环法。将2.5%的STMP水解液应用于人工龋损处，使部分脱矿的胶原基质磷酸化。一半的STMP处理样品是一步粘接的。粘附性和非粘附性渗透标本在含有聚丙烯酸(PAA)的波特兰水泥模拟体液系统中重新矿化，以稳定无定形磷酸钙作为纳米递归。术后4个月用微电脑断层扫描(Micro-CT)和透射电子显微镜(TEM)观察再矿化效果。在没有PAA和STMP作为仿生类似物的情况下(对照组)，无论皮损是否被粘合剂浸透，都没有再矿化。对于浸泡在含有PAA的模拟体液中的STMP处理的实验组，没有粘附剂渗透的试件比浸入粘附剂的试件更重的再矿化。对4个月后的Micro-CT数据的统计分析显示，非粘接组和粘接组在病变深度、病变表面的相对矿物质含量和ΔZ的变化方面均有显著差异(P<；0.05)。透射电子显微镜检查显示，非粘结剂组、粘结剂对照组和实验组在再矿化4个月后均出现胶原降解。使用STMP的仿生再矿化是一种很有前途的人工龋损再矿化方法，特别是在缺乏种子晶体的区域。未来的研究应该考虑在部分脱钙的胶原基质中加入基质金属蛋白酶抑制剂，以防止胶原在再矿化过程中的降解。
This study examined the use of sodium trimetaphosphate (STMP) as a biomimetic analog of matrix phosphoproteins for remineralization of artificial carious-affected dentin. Artificial carious lesions with lesion depths of 300±30 µm were created by pH-cycling. 2.5% hydrolyzed STMP was applied to the artificial carious lesions to phosphorylate the partially-demineralized collagen matrix. Half of the STMP-treated specimens were bonded with One-Step. The adhesive and non-adhesive infiltrated specimens were remineralized in a Portland cement-simulated body fluid system containing polyacrylic acid (PAA) to stabilize amorphous calcium phosphate as nanoprecursors. Micro-computed tomography (micro-CT) and transmission electron microscopy (TEM) were used to evaluate the results of remineralization after a 4-month period. In absence of PAA and STMP as biomimetic analogs (control groups), there was no remineralization irrespective of whether the lesions were infiltrated with adhesive. For the STMP-treated experimental groups immersed in PAA-containing simulated body fluid, specimens without adhesive infiltration were more heavily remineralized than those infiltrated with adhesive. Statistical analysis of the 4-month micro-CT data revealed significant differences in the lesion depth, relative mineral content along the lesion surface and changes in ΔZ between the non-adhesive and adhesive experimental groups (p<0.05 for all the three parameters). TEM examination indicated that collagen degradation occurred in both the non-adhesive and adhesive control and experimental groups after 4 months of remineralization. Biomimetic remineralization using STMP is a promising method to remineralize artificial carious lesions particularly in areas devoid of seed crystallites. Future studies should consider the incorporation of MMP-inhibitors within the partially-demineralized collagen matrix to prevent collagen degradation during remineralization.
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影响因子：
9.9
作者：
Liu, Y;Sethuraman, G;Grynpas, M
通讯作者：
Grynpas, M
影响因子：
1.8
作者：
KOUTSOUKOS, PG;NANCOLLAS, GH
通讯作者：
NANCOLLAS, GH
影响因子：
9.7
作者：
Kim, Young Kyung;Mai, Sui;Mazzoni, Annalisa;Liu, Yan;Tezvergil-Mutluay, Arzu;Takahashi, Kei;Zhang, Kai;Pashley, David H.;Tay, Franklin R.
通讯作者：
Tay, Franklin R.
DOI：
10.1111/j.1532-849x.2006.00079.x
发表时间：
2006-03-01
期刊：
Journal of prosthodontics : official journal of the American College of Prosthodontists
影响因子：
--
作者：
Haj-Ali, Reem;Walker, Mary;Spencer, Paulette
通讯作者：
Spencer, Paulette
DOI：
10.1016/0006-291x(81)91497-2
发表时间：
1981-01-01
影响因子：
3.1
作者：
KUBOKI, Y;TSUZAKI, M;MECHANIC, GL
通讯作者：
MECHANIC, GL