Separation of sub-micron particles from micron particles using acoustic fluid relocation combined with acoustophoresis.
Separation of sub-micron particles from micron particles using acoustic fluid relocation combined with acoustophoresis.
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DOI：
10.1007/s00216-018-1261-x
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发表时间：
2018-10
影响因子：
4.3
通讯作者：
Piyasena ME
中科院分区：
文献类型：
--
作者：
Gautam GP;Gurung R;Fencl FA;Piyasena ME
声泳作为一种温和、非接触、高通量的细胞和颗粒分离技术，越来越受到人们的关注。它可以方便地用于分离和富集大于2 μm的颗粒;然而，它在操纵小于2 μm的颗粒方面的用途有限。在这项工作中，我们提出了一种替代的方式使用声力操纵亚微米颗粒在连续流动的方式。已经表明，声力可以用来重新定位两个阻抗失配流体的平行层流流。我们展示了从微米颗粒分离的声电泳和声流体搬迁相结合。微米颗粒通过初级声学力聚焦到流动通道的中间，而亚微米颗粒通过由重新定位流体产生的拖曳力移动到侧面。我们使用由亚微米/微米颗粒、微米/微米颗粒和牛红细胞与E.杆菌通过对收集的流进行流式细胞术分析来确定颗粒富集的效率。研究表明，声流体再定位与声电泳相结合，可以在高流速下有效分离亚微米级颗粒和微米级颗粒，并可进一步实现对体积比大于10且较大粒径在10 μm左右的微米级颗粒二元混合物的分离。在可能关注声流和声阻抗差异的情况下，组合方法比声泳更适合使用。在共振声场存在的情况下，清洁的高密度流体（深灰色）和低密度样品流体被重新定位。在这个过程中，大肠杆菌与红细胞（RBC）分离。
Acoustophoresis has gained increasing attention as a gentle, non-contact, and high throughput cell and particle separation technique. It is conveniently used to isolate and enrich particles that are greater than 2 μm; however, its use in manipulating particles smaller than 2 μm is limited. In this work, we present an alternative way of using acoustic forces to manipulate sub-micrometer particles in continuous flow fashion. It has been shown that acoustic forces can be employed to relocate parallel laminar flow streams of two impedance mis-matched fluids. We demonstrate the separation of sub-micron particles from micron particles by the combination of acoustophoresis and acoustic fluid relocation. The micron particles are focused into the middle of the flow channel via primary acoustic forces while sub-micron particles are moved to the side via drag forces created by the relocating fluid. We demonstrate the proof of the concept using binary mixtures of particles comprised of sub-micron/micron particles, micron/micron particles, and bovine red blood cells with E. coli. The efficiency of the particle enrichment is determined via flow cytometry analysis of the collected streams. This study demonstrates that by combining acoustic fluid relocation with acoustophoresis, sub-micron particles can be effectively separated from micron particles at high flow rates and it can be further implemented to separate binary mixtures of micron particles if the volumetric ratio of two particles is greater than 10 and the larger particle diameter is about 10 μm. The combined method is more appropriate to use than acoustophoresis in situations where acoustic streaming and differences in acoustic impedance of fluids can be concerns. In the presence of a resonance acoustic field, the clean high-density fluid (dark grey) and the low-density sample fluid are relocated. During this process, E coli are separated from the red blood cells (RBC).
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影响因子：
7.4
作者：
Piyasena, Menake E.;Suthanthiraraj, Pearlson P. Austin;Applegate, Robert W., Jr.;Goumas, Andrew M.;Woods, Travis A.;Lopez, Gabriel P.;Graves, Steven W.
通讯作者：
Graves, Steven W.
影响因子：
14
作者：
Didar, Tohid Fatanat;Li, Kebin;Tabrizian, Maryam
通讯作者：
Tabrizian, Maryam
影响因子：
2.2
作者：
Lamparski, HG;Metha-Damani, A;Le Pecq, JB
通讯作者：
Le Pecq, JB
影响因子：
10.8
作者：
Akbulut, Ozge;Mace, Charles R.;Whitesides, George M.
通讯作者：
Whitesides, George M.
影响因子：
6.1
作者：
Chen C;Skog J;Hsu CH;Lessard RT;Balaj L;Wurdinger T;Carter BS;Breakefield XO;Toner M;Irimia D
通讯作者：
Irimia D