Experimental infection of Balb/c nude mice with Hepatitis E virus.
Experimental infection of Balb/c nude mice with Hepatitis E virus.
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DOI：
10.1186/1471-2334-9-93
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发表时间：
2009-06-13
影响因子：
3.7
通讯作者：
Hua X
中科院分区：
文献类型：
--
作者：
Huang F;Zhang W;Gong G;Yuan C;Yan Y;Yang S;Cui L;Zhu J;Yang Z;Hua X
据报道，几种动物物种可以作为戊型肝炎病毒（HEV），一种人畜共患病原体的水库。在包括啮齿动物在内的多种动物中已检测到戊型肝炎病毒及其抗体。猪和大鼠的HEV模型已经建立，但裸鼠尚未开发。Balb/c裸小鼠经口和静脉注射猪戊型肝炎病毒以确保感染。研究中还包括阴性对照组和实验接触组小鼠。取各组小鼠的肝、脾、肾、空肠、回肠、盲肠和结肠进行RT-nPCR检测、间接免疫荧光观察和组织病理学检查。用酶联免疫吸附试验（ELISA）检测裸鼠血清中抗-HEVIgG。同时测定裸鼠血清中的肝酶活性，包括丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和碱性磷酸酶（ALP），以及总胆红素（TBIL）。用间接免疫荧光法和RT-nPCR法在所有接种的裸鼠和1只接触暴露的裸鼠的肝、脾、肾、空肠、回肠和结肠中检测到HEV抗原和HEV RNA。在这些小鼠的肝脏和脾脏中观察到组织学变化。感染小鼠血清中AST、ALP和抗HEV IgG水平升高。接触组小鼠肝脏出现明显的组织病理学损伤。猪源戊型肝炎病毒能很容易地感染裸鼠。因此，裸鼠可能是研究HEV发病机制的一种有用的动物模型。
Several animal species can reportedly act as reservoirs for Hepatitis E virus (HEV), a zoonotic pathogen. HEV and antibody to the virus have been detected in a variety of animals including rodents. Pig and rat models for HEV have been established for HEV, but a nude mouse has not yet been developed. Balb/c nude mice were inoculated with swine HEV, both orally and via intravenous injection to insure infection. Negative control and experimental contact-exposed groups of mice were also included in the study. The liver, spleen, kidney, jejunum, ileum, cecum and colon of each mouse from all three groups were collected for reverse transcription nested polymerase chain reaction (RT-nPCR) detection, indirect immunofluorescence observation and histopathologic examination. The sera from nude mice were tested for anti-HEV IgG by enzyme linked immunosorbent assay (ELISA). Activities of liver enzymes, including alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP), as well as total bilirubin (TBIL) were also measured in the sera of the nude mice. HEV antigens and HEV RNA were detected in liver, spleen, kidney, jejunum, ileum and colon both by indirect immunofluorescence and by RT-nPCR in all of the inoculated and in one of the contact-exposed nude mice. Histopathological changes were observed in the liver and spleen of these mice. Infected mice showed increased levels of AST, ALP, and anti-HEV IgG in sera. The livers of contact-exposed mice showed obvious histopathological damage. Nude mice could be readily infected by HEV isolated from pigs. The nude mouse may therefore be a useful animal model for studying the pathogenesis of HEV.
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影响因子：
3.8
作者：
Tanaka, Toshinori;Takahashi, Masaharu;Okamoto, Hiroaki
通讯作者：
Okamoto, Hiroaki
影响因子：
3.1
作者：
Kasorndorkbua, C;Halbur, PG;Meng, XJ
通讯作者：
Meng, XJ
影响因子：
5.4
作者：
Meng, XJ;Halbur, PG;Emerson, SU
通讯作者：
Emerson, SU
影响因子：
12.7
作者：
Feagins, A. R.;Opriessnig, T.;Huang, Y. W.;Halbur, P. G.;Meng, X. J.
通讯作者：
Meng, X. J.
影响因子：
9.4
作者：
Halbur, PG;Kasorndorkbua, C;Meng, XJ
通讯作者：
Meng, XJ