Maize germinal cell initials accommodate hypoxia and precociously express meiotic genes.
Maize germinal cell initials accommodate hypoxia and precociously express meiotic genes.
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DOI：
10.1111/tpj.12414
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发表时间：
2014-02
期刊：
影响因子：
--
通讯作者：
Walbot V
中科院分区：
文献类型：
--
作者：
Kelliher T;Walbot V
在开花植物中，花药是新生生殖细胞特化、雄性减数分裂和花粉发育的场所。不典型的是，花药没有分生组织。相反，生殖细胞和体细胞类型都与聚集在花药裂片中的花干细胞不同。为了更好地了解花药细胞命运的特性，并为生殖生物界提供资源，我们在获得命运后36小时内从玉米花药中分离了生发和体细胞起始序列，鉴定了815个特异的和1714个显著富含的生发转录本，以及2439个显著富含的体细胞转录本和2112个显著富含的体细胞转录本。为了阐明参与细胞分化的转录本，我们将这些转录本与FATE指定之前的花药原基和在FATE指定的第一步中被阻止的msca1花药进行了对比，因此缺乏正常的细胞类型。精制的细胞特异性图谱表明，生殖细胞和体细胞群体分化迅速，并表达独特的转录因子集；通过原位杂交验证了转录定位的一个子集。令人惊讶的是，开始五天有丝分裂的生发初始显著富含在减数分裂过程中分类的>；100转录本，包括重组和突触，以及参与RNA代谢、氧化还原动态平衡和细胞质ATP生成的基因集。生发初期减数分裂特异基因的丰富挑战了当前的观点，即有丝分裂向减数分裂的转变发生在S减数分裂前阶段发育的较晚阶段。细胞质能量产生基因的表达表明，雄性生发细胞通过将碳从线粒体呼吸转移到避免产生活性氧物种(ROS)的替代途径来适应缺氧。
In flowering plants, anthers are the site of de novo germinal cell specification, male meiosis, and pollen development. Atypically, anthers lack a meristem. Instead, both germinal and somatic cell types differentiate from floral stem cells packed into anther lobes. To better understand anther cell fate specification and to provide a resource for the reproductive biology community, we isolated cohorts of germinal and somatic initials from maize anthers within 36 hours of fate acquisition, identifying 815 specific and 1714 significantly enriched germinal transcripts, plus 2439 specific and 2112 significantly enriched somatic transcripts. To clarify transcripts involved in cell differentiation, we contrasted these profiles to anther primordia prior to fate specification and to msca1 anthers arrested in the first step of fate specification and hence lacking normal cell types. The refined cell-specific profiles demonstrate that both germinal and somatic cell populations differentiate quickly and express unique transcription factor sets; a subset of transcript localizations were validated by in situ hybridization. Surprisingly, germinal initials starting five days of mitotic divisions were significantly enriched in >100 transcripts classified in meiotic processes including recombination and synapsis, along with gene sets involved in RNA metabolism, redox homeostasis, and cytoplasmic ATP generation. Enrichment of meiotic-specific genes in germinal initials challenges current dogma that the mitotic to meiotic transition occurs later in development during pre-meiotic S phase. Expression of cytoplasmic energy generation genes suggests that male germinal cells accommodate hypoxia by diverting carbon away from mitochondrial respiration into alternative pathways that avoid producing reactive oxygen species (ROS).
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DOI：
10.1126/science.1229277
发表时间：
2013-01-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hackett JA;Sengupta R;Zylicz JJ;Murakami K;Lee C;Down TA;Surani MA
通讯作者：
Surani MA
影响因子：
64.5
作者：
Calarco JP;Borges F;Donoghue MT;Van Ex F;Jullien PE;Lopes T;Gardner R;Berger F;Feijó JA;Becker JD;Martienssen RA
通讯作者：
Martienssen RA
影响因子：
4
作者：
Golubovskaya, Inna N.;Hamant, Olivier;Cande, W. Zacheus
通讯作者：
Cande, W. Zacheus
影响因子：
7.2
作者：
Feng, Baomin;Lu, Dihong;Ma, Hong
通讯作者：
Ma, Hong
影响因子：
27.5
作者：
Ding, Jihua;Shen, Jianqiang;Zhang, Qifa
通讯作者：
Zhang, Qifa