Cytokeratin 17 mRNA as a prognostic marker of oral squamous cell carcinoma.
Cytokeratin 17 mRNA as a prognostic marker of oral squamous cell carcinoma.
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DOI：
10.3892/ol.2017.7066
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发表时间：
2017-12
期刊：
影响因子：
2.9
通讯作者：
Nakamura S
中科院分区：
文献类型：
--
作者：
Kitamura R;Toyoshima T;Tanaka H;Kawano S;Matsubara R;Goto Y;Jinno T;Maruse Y;Oobu K;Nakamura S
尽管诊断和治疗取得了进步，但口腔鳞状细胞癌(OSCC)的5年生存率仍保持在70%-80%之间，原因是复发和继发性颈淋巴转移。术后很难发现这些复发和转移，因此，建议仔细随访。细胞角蛋白(CKs)是细胞骨架的中间丝，是口腔鳞癌的候选预后生物标志物，因为它们在口腔鳞状细胞癌中过度表达。本研究采用逆转录定量聚合酶链式反应(RT-Q-PCR)检测外周血单个核细胞(PBMC)中3种CK基因(CK17、CK19、CK20)转录本的相对水平。这项研究包括19名口腔鳞癌患者手术前和手术后的PBMC样本。预后不良组13例中有10例术后CK17基因表达较术前降低，而预后不良组6例中仅有3例术后CK17基因表达降低。这一差异具有统计学意义(P<；0.01)。术后CK17基因表达降低组的无瘤生存率显著高于CK17基因表达升高组(P<；0.01)，但两组总体生存率差异无统计学意义。口腔鳞癌患者外周血单个核细胞均未见CK19和CK20的表达。总之，CK17mRNA的表达可能是口腔鳞癌的一个有用的预后生物标志物。
Despite diagnostic and therapeutic advances, the 5-year survival rate of oral squamous cell carcinoma (OSCC) remains between 70–80% due to recurrences and secondary metastases to cervical lymph nodes. It is difficult to find these recurrences and metastases postoperatively, thus, careful follow-up is recommended. Cytokeratins (CKs) are intermediate filaments of the cytoskeleton and candidate prognostic biomarkers for OSCC, as they are overexpressed in OSCC compared with normal mucosa. The aim of the present study was to determine the relative levels of occurrence of 3 CK mRNA (CK17, CK19, CK20) transcripts in peripheral blood mononuclear cells (PBMC) using reverse transcription-quantitative polymerase chain reaction. The study comprised pre- and post-operative PBMC samples from 19 OSCC patients. In the good-prognosis group, 10 of 13 patients demonstrated reduced CK17 mRNA expression post-operatively, compared with pre-operative samples, conversely, only 3 of 6 patients in the poor-prognosis group had reduced post-operative CK17 mRNA expression. This difference was statistically significant (P<0.01). The disease-free survival rate of the group with reduced post-operative CK17 mRNA expression was significantly increased compared with the elevated CK17 mRNA group (P<0.01); however, the overall survival rates of the two groups were not significantly different. Neither CK19 mRNA nor CK20 mRNA were significantly expressed in the PBMC of OSCC patients. Overall, CK17 mRNA expression may be a useful prognostic biomarker for OSCC.
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影响因子：
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作者：
Davelaar, Elvira M.;van de Lande, Jonas;Kenemans, Peter
通讯作者：
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DOI：
10.1016/s0901-5027(05)80490-4
发表时间：
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