Sterilising effects of pyriproxyfen on Anopheles arabiensis and its potential use in malaria control.
Sterilising effects of pyriproxyfen on Anopheles arabiensis and its potential use in malaria control.
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DOI：
10.1186/1756-3305-6-144
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发表时间：
2013-05-17
影响因子：
3.2
通讯作者：
Majambere S
中科院分区：
文献类型：
--
作者：
Harris C;Lwetoijera DW;Dongus S;Matowo NS;Lorenz LM;Devine GJ;Majambere S
抗药性对目前的病媒控制运动构成重大威胁。因此，对具有新颖作用模式的抑制剂的需求很高。吡丙醚（PPF）是一种传统的杀蚊蛹剂，具有独特的作用模式，在直接接触时也能使成年蚊子（无法产生可存活的后代）绝育。然而，与蚊子吸血有关的PPF暴露时间对消毒有重要影响。本研究调查了不同的血液喂养和PPF暴露时间之间的关系，以确定PPF灭菌在控制阿拉伯按蚊中的潜力。研究了四种治疗方案：PPF暴露前3天饲喂的血液（A）、PPF暴露前1天饲喂的血液（B）、PPF暴露后1天饲喂的血液（C）和PPF暴露后3天饲喂的血液（D）对产蛋的影响（繁殖力）和可存活后代的产生（生育力），而在没有血餐的情况下研究了PPF暴露对蚊子存活的影响。所有方案和存活研究均通过瓶试验将蚊子暴露于PPF，剂量为3 mg AI/m2，持续30分钟。在PPF暴露前一天吸血的雌性蚊子（方案B）在该生殖营养周期期间没有产生可存活的后代（生育力降低100%）。所有其他处理均无显著影响。观察到的生殖力和生育力降低是由卵子保留引起的（方案B中97%的卵子保留，即在卵巢中产生但未产下，p = 0.0004）。这些残留的卵中有一些是变形的。在没有血餐的情况下，PPF暴露对蚊子的存活没有影响。这里提出的结果表明，使用PPF对成年疟疾病媒进行绝育可以成为疟疾控制策略的一部分，利用蚊子缺乏对PPF的抗性及其独特的作用模式。我们建议，针对静止的蚊子，这是非常敏感的PPF在低剂量，是开发这种控制工具的最佳方向。
Insecticide resistance poses a major threat to current vector control campaigns. Insecticides with novel modes of action are therefore in high demand. Pyriproxyfen (PPF), a conventional mosquito pupacide, has a unique mode of action that also sterilises adult mosquitoes (unable to produce viable offspring) upon direct contact. However, the timing of PPF exposure in relation to when mosquitoes take a blood meal has an important impact on that sterilisation. This study investigated the relationship between different blood feeding and PPF exposure timings to determine the potential of PPF sterilisation in controlling Anopheles arabiensis. Four treatment regimens were investigated: blood fed three days before PPF exposure (A), blood fed one day before PPF exposure (B), blood fed one day after PPF exposure (C) and blood fed three days after PPF exposure (D) for their impact on egg laying (fecundity) and the production of viable offspring (fertility), while the impact of PPF exposure on mosquito survival was investigated in the absence of a blood meal. All regimens and the survival study exposed mosquitoes to PPF via the bottle assay at 3 mg AI/m2 for 30 minutes. Female mosquitoes that blood-fed one day prior to PPF exposure (regimen B), produced no viable offspring during that gonotrophic cycle (100% reduction in fertility). All other treatments had no significant effect. The observed reductions in fecundity and fertility were caused by the retention of eggs (97% of eggs retained, i.e. produced in the ovaries but not laid, in regimen B, p = 0.0004). Some of these retained eggs were deformed in shape. PPF exposure on mosquito survival in the absence of a blood meal was found to have no effect. The results presented here suggest that sterilising adult malaria vectors using PPF could form part of a malaria control strategy, taking advantage of the lack of reported resistance to PPF in mosquitoes and its unique mode of action. We propose that targeting resting mosquitoes, which are highly susceptible to PPF at low doses, is the optimal direction for developing this control tool.
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相对于单独的方法，相对于单独的两种方法，对长期杀虫剂处理的网和室内残留喷涂的组合的比较现场评估，在主载体是Anopheles Aropheles Arabiensis的区域中预防疟疾。
影响因子：
3.2
作者：
Okumu FO;Mbeyela E;Lingamba G;Moore J;Ntamatungiro AJ;Kavishe DR;Kenward MG;Turner E;Lorenz LM;Moore SJ
通讯作者：
Moore SJ
影响因子：
3
作者：
Russell, Tanya L.;Lwetoijera, Dickson W.;Killeen, Gerry F.
通讯作者：
Killeen, Gerry F.
DOI：
10.1073/pnas.0901369106
发表时间：
2009-07-14
影响因子：
11.1
作者：
Devine, Gregor J.;Zamora Perea, Elvira;Morrison, Amy C.
通讯作者：
Morrison, Amy C.
DOI：
10.4269/ajtmh.1972.21.755
发表时间：
1972-01-01
影响因子：
3.3
作者：
ELLIOTT, R
通讯作者：
ELLIOTT, R
影响因子：
2.7
作者：
Jambulingam, P.;Sadanandane, C.;Zaim, M.
通讯作者：
Zaim, M.